5y85: Difference between revisions
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The | ==Structure-based Insights into Self-Cleavage by a Four-way Junctional Twister-Sister Ribozyme== | ||
<StructureSection load='5y85' size='340' side='right' caption='[[5y85]], [[Resolution|resolution]] 2.00Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[5y85]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5Y85 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5Y85 FirstGlance]. <br> | |||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5y85 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5y85 OCA], [http://pdbe.org/5y85 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5y85 RCSB], [http://www.ebi.ac.uk/pdbsum/5y85 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5y85 ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Here we report on the crystal structure and cleavage assays of a four-way junctional twister-sister self-cleaving ribozyme. Notably, 11 conserved spatially separated loop nucleotides are brought into close proximity at the ribozyme core through long-range interactions mediated by hydrated Mg2+ cations. The C62-A63 step at the cleavage site adopts a splayed-apart orientation, with flexible C62 directed outwards, whereas A63 is directed inwards and anchored by stacking and hydrogen-bonding interactions. Structure-guided studies of key base, sugar, and phosphate mutations in the twister-sister ribozyme, suggest contributions to the cleavage chemistry from interactions between a guanine at the active site and the non-bridging oxygen of the scissile phosphate, a feature found previously also for the related twister ribozyme. Our four-way junctional pre-catalytic structure differs significantly in the alignment at the cleavage step (splayed-apart vs. base-stacked) and surrounding residues and hydrated Mg2+ ions relative to a reported three-way junctional pre-catalytic structure of the twister-sister ribozyme. | |||
Structure-based insights into self-cleavage by a four-way junctional twister-sister ribozyme.,Zheng L, Mairhofer E, Teplova M, Zhang Y, Ma J, Patel DJ, Micura R, Ren A Nat Commun. 2017 Oct 30;8(1):1180. doi: 10.1038/s41467-017-01276-y. PMID:29081514<ref>PMID:29081514</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
<div class="pdbe-citations 5y85" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Micura, R L]] | |||
[[Category: Ren, A]] | [[Category: Ren, A]] | ||
[[Category: Zheng, L]] | [[Category: Zheng, L]] | ||
[[Category: Catalysis]] | |||
[[Category: Riboyzme]] | |||
[[Category: Rna]] | |||
[[Category: Self-cleaving]] | |||
[[Category: Twister-sister]] | |||
Revision as of 07:46, 22 November 2017
Structure-based Insights into Self-Cleavage by a Four-way Junctional Twister-Sister Ribozyme
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