4cne

Crystal structure of E.coli TrmJ in complex with S-adenosyl-L- homocysteine

spinqualitylabelsall models 4cne, resolution 1.90Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 4cne is a 2 chain structure. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: PEG, SAH Related: 4cnd, 4cnf, 4cng Activity: tRNA (cytidine(32)/uridine(32)-2'-O)-methyltransferase, with EC number 2.1.1.200 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function [TRMJ_ECOLI] Catalyzes the formation of 2'O-methylated cytidine (Cm32) or 2'O-methylated uridine (Um32) at position 32 in tRNA. Publication Abstract from PubMed The 2'-O-methylation of the nucleoside at position 32 of tRNA is found in organisms belonging to the three domains of life. Unrelated enzymes catalyzing this modification in Bacteria (TrmJ) and Eukarya (Trm7) have already been identified, but until now, no information is available for the archaeal enzyme. In this work we have identified the methyltransferase of the archaeon Sulfolobus acidocaldarius responsible for the 2'-O-methylation at position 32. This enzyme is a homolog of the bacterial TrmJ. Remarkably, both enzymes have different specificities for the nature of the nucleoside at position 32. While the four canonical nucleosides are substrates of the Escherichia coli enzyme, the archaeal TrmJ can only methylate the ribose of a cytidine. Moreover, the two enzymes recognize their tRNA substrates in a different way. We have solved the crystal structure of the catalytic domain of both enzymes to gain better understanding of these differences at a molecular level. Characterization of two homologous 2'-O-methyltransferases showing different specificities for their tRNA substrates.,Somme J, Van Laer B, Roovers M, Steyaert J, Versees W, Droogmans L RNA. 2014 Jun 20. PMID:24951554 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also TRNA methyltransferase

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