4zty

Neurospora crassa cobalamin-independent methionine synthase complexed with Cd2+

spinqualitylabelsall models 4zty, resolution 1.88Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 4zty is a 1 chain structure with sequence from Neurospora crassa. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: CD, GOL, NO3, TRS Related: 4ztx Activity: 5-methyltetrahydropteroyltriglutamate--homocysteine S-methyltransferase, with EC number 2.1.1.14 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed Two families of methionine synthases, distinct in catalytic and structural features, have been encountered: MetH, the cobalamin-dependent enzyme and MetE, the cobalamin-independent form. The MetE family is of mechanistic interest due to the chemically challenging nature of the reaction and is a potential target for antifungal therapeutics since the human genome encodes only MetH. Here we report the identification, purification, and crystal structure of MetE from the filamentous fungus Neurospora crassa (ncMetE). ncMetE was highly thermostable and crystallized readily, making it ideal for study. Crystal structures of native ncMetE in complex with either Zn2+or Cd2+ were solved at resolution limits of 2.10 A and 1.88 A, respectively. The monomeric protein contains two domains, each containing a (betaalpha)8 barrel core, and a long alpha-helical segment spans the length of the protein, connecting the domains. Zn2+ bound in the C-terminal domain exhibits tetrahedral coordination with the side chains of His 652, Cys 654, Glu 676 and Cys 737. A Cd2+ replete structure revealed a supermetalated enzyme and demonstrated the inate flexibility of the metal binding site. An extensive analysis of sequence conservation within the MetE family identified 57 highly conserved residues and 60 additional residues that were conserved in all fungal sequences examined. Fungal cobalamin-independent methionine synthase: Insights from the model organism, Neurospora crassa.,Wheatley RW, Ng KK, Kapoor M Arch Biochem Biophys. 2015 Nov 30;590:125-137. doi: 10.1016/j.abb.2015.11.037. PMID:26657067 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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