5f3j

Crystal structure of DBP in complex with inhibitory monoclonal antibody 2D10

spinqualitylabelsall models 5f3j, resolution 4.00Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 5f3j is a 4 chain structure with sequence from Lk3 transgenic mice and Plavs. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Gene: PVDR (PLAVS) Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function [PVDR_PLAVS] Binds to the human erythrocytes Duffy blood group determinant. Publication Abstract from PubMed Plasmodium vivax Duffy Binding Protein (PvDBP) is the most promising vaccine candidate for P. vivax malaria. The polymorphic nature of PvDBP induces strain-specific immune responses, however, and the epitopes of broadly neutralizing antibodies are unknown. These features hamper the rational design of potent DBP-based vaccines and necessitate the identification of globally conserved epitopes. Using X-ray crystallography, small-angle X-ray scattering, hydrogen-deuterium exchange mass spectrometry, and mutational mapping, we have defined epitopes for three inhibitory mAbs (mAbs 2D10, 2H2, and 2C6) and one noninhibitory mAb (3D10) that engage DBP. These studies expand the currently known inhibitory epitope repertoire by establishing protective motifs in subdomain three outside the receptor-binding and dimerization residues of DBP, and introduce globally conserved protective targets. All of the epitopes are highly conserved among DBP alleles. The identification of broadly conserved epitopes of inhibitory antibodies provides critical motifs that should be retained in the next generation of potent vaccines for P. vivax malaria. Broadly neutralizing epitopes in the Plasmodium vivax vaccine candidate Duffy Binding Protein.,Chen E, Salinas ND, Huang Y, Ntumngia F, Plasencia MD, Gross ML, Adams JH, Tolia NH Proc Natl Acad Sci U S A. 2016 May 18. pii: 201600488. PMID:27194724 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Antibody 3D structures Monoclonal Antibodies 3D structures

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