5cw0

Investigation of RNA structure in satellite panicum mosaic virus

spinqualitylabelsall models 5cw0, resolution 4.60Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 5cw0 is a 1 chain structure with sequence from Satellite panicum mosaic virus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Related: 1stm Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed Three new crystal forms of satellite panicum mosaic virus (SPMV) were grown and their structures solved from X-ray diffraction data using molecular replacement techniques. The crystals were grown under conditions of pH and ionic strength that were appreciably different then those used for the original structure determination. In rhombohedral crystals grown at pH 8.5 and low ionic strength PEG 3350 solutions, Fourier syntheses revealed segments, ten amino acid residues long, of amino-terminal polypeptides not previously seen, as well as masses of electron density within concavities on the interior of the capsid, which appeared in the neighborhoods of icosahedral five- and threefold axes. The densities were compatible with secondary structural domains of RNA, and they included a segment of double helical RNA of about four to five base pairs oriented, at least approximately, along the fivefold axes. The distribution of RNA observed for SPMV appears to be distinctly different than the encapsidated nucleic acid conformation previously suggested for another satellite virus, satellite tobacco mosaic virus. This study further shows that analysis of viruses in crystals grown under different chemical conditions may reveal additional information regarding the structure of encapsidated RNA. Investigation of RNA structure in satellite panicum mosaic virus.,Makino DL, Day J, Larson SB, McPherson A Virology. 2006 Aug 1;351(2):420-31. Epub 2006 May 4. PMID:16677679 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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