Pyruvate phosphate dikinase: Difference between revisions
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<p align="center"><html5media width=" | <p align="center"><html5media width="500" height="281" frameborder="0" allowfullscreen>https://www.youtube.com/embed/hxuBouGfs_4</html5media></p> | ||
'''''The movie''' depicts the catalytic reaction involving 3 in-line phosphotransfers & the accompanied protein conformational transitions. Model based on crystal structures of PPDK from '''Clostridium symbiosum''' in the 2 extreme conformational states shown to the left and of complexes bound to substrate analogs, phosphonopyruvate and 5'-adenylyl-β,γ-imidodiphosphate (AMPPNP). The nucleotide binding subdomains are colored green & blue, PEP binding domain colored cyan, His-domain colored yellow, and linker segments that connect the His-domain to the partner domains colored red. Ligands and the catalytic histidine are depicted in stick models with the atomic color scheme: '''''C''''' – gray, '''''N''''' – blue, '''''O''''' – red, '''''P''''' – green, '''''Mg''''' – magenta. Note that the reaction progresses in the movie in the reverse direction; steps 3 and 2 occur first followed by step 1. The movie was created by Kap Lim and Osnat Herzberg''<br> | '''''The movie''' depicts the catalytic reaction involving 3 in-line phosphotransfers & the accompanied protein conformational transitions. Model based on crystal structures of PPDK from '''Clostridium symbiosum''' in the 2 extreme conformational states shown to the left and of complexes bound to substrate analogs, phosphonopyruvate and 5'-adenylyl-β,γ-imidodiphosphate (AMPPNP). The nucleotide binding subdomains are colored green & blue, PEP binding domain colored cyan, His-domain colored yellow, and linker segments that connect the His-domain to the partner domains colored red. Ligands and the catalytic histidine are depicted in stick models with the atomic color scheme: '''''C''''' – gray, '''''N''''' – blue, '''''O''''' – red, '''''P''''' – green, '''''Mg''''' – magenta. Note that the reaction progresses in the movie in the reverse direction; steps 3 and 2 occur first followed by step 1. The movie was created by Kap Lim and Osnat Herzberg''<br> | ||
<br>You may also [http://youtu.be/hxuBouGfs_4 download] the full High Resolution video. | <br>You may also [http://youtu.be/hxuBouGfs_4 download] the full High Resolution video. | ||
