1zlb: Difference between revisions
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'''Crystal structure of catalytically-active phospholipase A2 in the absence of calcium''' | '''Crystal structure of catalytically-active phospholipase A2 in the absence of calcium''' | ||
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Insights into metal ion binding in phospholipases A2: ultra high-resolution crystal structures of an acidic phospholipase A2 in the Ca2+ free and bound states., Murakami MT, Gabdoulkhakov A, Genov N, Cintra AC, Betzel C, Arni RK, Biochimie. 2006 May;88(5):543-9. Epub 2005 Dec 5. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16376474 16376474] | Insights into metal ion binding in phospholipases A2: ultra high-resolution crystal structures of an acidic phospholipase A2 in the Ca2+ free and bound states., Murakami MT, Gabdoulkhakov A, Genov N, Cintra AC, Betzel C, Arni RK, Biochimie. 2006 May;88(5):543-9. Epub 2005 Dec 5. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16376474 16376474] | ||
[[Category: Bothrops jararacussu]] | [[Category: Bothrops jararacussu]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Arni, R K.]] | [[Category: Arni, R K.]] | ||
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[[Category: Genov, N.]] | [[Category: Genov, N.]] | ||
[[Category: Murakami, M T.]] | [[Category: Murakami, M T.]] | ||
[[Category: | [[Category: Asp49-pla2]] | ||
[[Category: | [[Category: Snake venom]] | ||
[[Category: | [[Category: Toxin]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 17:46:00 2008'' | |||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | |||
Revision as of 14:46, 3 May 2008
Crystal structure of catalytically-active phospholipase A2 in the absence of calcium
Overview
The electrophile Ca(2+) is an essential multifunctional co-factor in the phospholipase A(2) mediated hydrolysis of phospholipids. Crystal structures of an acidic phospholipase A(2) from the venom of Bothrops jararacussu have been determined both in the Ca(2+) free and bound states at 0.97 and 1.60 A resolutions, respectively. In the Ca(2+) bound state, the Ca(2+) ion is penta-coordinated by a distorted pyramidal cage of oxygen and nitrogen atoms that is significantly different to that observed in structures of other Group I/II phospholipases A(2). In the absence of Ca(2+), a water molecule occupies the position of the Ca(2+) ion and the side chain of Asp49 and the calcium-binding loop adopts a different conformation.
About this Structure
1ZLB is a Single protein structure of sequence from Bothrops jararacussu. Full crystallographic information is available from OCA.
Reference
Insights into metal ion binding in phospholipases A2: ultra high-resolution crystal structures of an acidic phospholipase A2 in the Ca2+ free and bound states., Murakami MT, Gabdoulkhakov A, Genov N, Cintra AC, Betzel C, Arni RK, Biochimie. 2006 May;88(5):543-9. Epub 2005 Dec 5. PMID:16376474 Page seeded by OCA on Sat May 3 17:46:00 2008
