1zue: Difference between revisions
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'''Revised Solution Structure of DLP-2''' | '''Revised Solution Structure of DLP-2''' | ||
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==About this Structure== | ==About this Structure== | ||
1ZUE is a [[Single protein]] structure | 1ZUE is a [[Single protein]] structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZUE OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: Torres, A M.]] | [[Category: Torres, A M.]] | ||
[[Category: Tsampazi, C.]] | [[Category: Tsampazi, C.]] | ||
[[Category: | [[Category: Antiparallel beta-sheet]] | ||
[[Category: | [[Category: Helix]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 18:05:12 2008'' | |||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | |||
Revision as of 15:05, 3 May 2008
Revised Solution Structure of DLP-2
Overview
The recent discovery that the natriuretic peptide OvCNPb (Ornithorhynchus venom C-type natriuretic peptide B) from platypus (Ornithorynchus anatinus) venom contains a D-amino acid residue suggested that other D-amino-acid-containing peptides might be present in the venom. In the present study, we show that DLP-2 (defensin-like peptide-2), a 42-amino-acid residue polypeptide in the platypus venom, also contains a D-amino acid residue, D-methionine, at position 2, while DLP-4, which has an identical amino acid sequence, has all amino acids in the L-form. These findings were supported further by the detection of isomerase activity in the platypus gland venom extract that converts DLP-4 into DLP-2. In the light of this new information, the tertiary structure of DLP-2 was recalculated using a new structural template with D-Met2. The structure of DLP-4 was also determined in order to evaluate the effect of a D-amino acid at position 2 on the structure and possibly to explain the large retention time difference observed for the two molecules in reverse-phase HPLC. The solution structures of the DLP-2 and DLP-4 are very similar to each other and to the earlier reported structure of DLP-2, which assumed that all amino acids were in the L-form. Our results suggest that the incorporation of the D-amino acid at position 2 has minimal effect on the overall fold in solution.
About this Structure
1ZUE is a Single protein structure. Full crystallographic information is available from OCA.
Reference
D-amino acid residue in a defensin-like peptide from platypus venom: effect on structure and chromatographic properties., Torres AM, Tsampazi C, Geraghty DP, Bansal PS, Alewood PF, Kuchel PW, Biochem J. 2005 Oct 15;391(Pt 2):215-20. PMID:16033333 Page seeded by OCA on Sat May 3 18:05:12 2008
