4pn1

Structure of S. pombe Pct1 RNA triphosphatase in complex with the Spt5 CTD

spinqualitylabelsall models 4pn1, resolution 2.80Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 4pn1 is a 8 chain structure with sequence from Cbs 356. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: EDO, FMT Related: 4pn0 Gene: pct1 (CBS 356) Activity: Hydrolase, with EC number 3.1.3.33 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function [CET1_SCHPO] First step of mRNA capping. Converts the 5'-triphosphate end of a nascent mRNA chain into a diphosphate end. Publication Abstract from PubMed mRNA capping enzymes are directed to nascent RNA polymerase II (Pol2) transcripts via interactions with the carboxy-terminal domains (CTDs) of Pol2 and transcription elongation factor Spt5. Fission yeast RNA triphosphatase binds to the Spt5 CTD, comprising a tandem repeat of nonapeptide motif TPAWNSGSK. Here we report the crystal structure of a Pct1.Spt5-CTD complex, which revealed two CTD docking sites on the Pct1 homodimer that engage TPAWN segments of the motif. Each Spt5 CTD interface, composed of elements from both subunits of the homodimer, is dominated by van der Waals contacts from Pct1 to the tryptophan of the CTD. The bound CTD adopts a distinctive conformation in which the peptide backbone makes a tight U-turn so that the proline stacks over the tryptophan. We show that Pct1 binding to Spt5 CTD is antagonized by threonine phosphorylation. Our results fortify an emerging concept of an "Spt5 CTD code" in which (i) the Spt5 CTD is structurally plastic and can adopt different conformations that are templated by particular cellular Spt5 CTD receptor proteins; and (ii) threonine phosphorylation of the Spt5 CTD repeat inscribes a binary on-off switch that is read by diverse CTD receptors, each in its own distinctive manner. Fission yeast RNA triphosphatase reads an Spt5 CTD code.,Doamekpor SK, Schwer B, Sanchez AM, Shuman S, Lima CD RNA. 2014 Nov 20. PMID:25414009 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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