Sandbox k11v: Difference between revisions

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[[Image:Rosetta_image.jpg | thumb | 300px | right | Fig. 1 : Ribbon diagram of a single subunit of ABC (16), colored by propensity to form amyloid, with red being the highest and blue the lowest propensity. The segment from residue 90 to 100, termed K11V, forms the cylindrin. ]]
[[Image:Rosetta_image.jpg | thumb | 300px | right | Fig. 1 : Ribbon diagram of a single subunit of ABC (16), colored by propensity to form amyloid, with red being the highest and blue the lowest propensity. The segment from residue 90 to 100, termed K11V, forms the cylindrin. ]]


The entire 11-residue segment KVKVLGDVIEV forms a <scene name='77/771966/K11v_in_black/1'>hairpin loop</scene> in the 3D structure of ABC.The six residue segment GDVIEV ,termed <scene name='77/771966/Gdviev_black/1'>G6V</scene>,forms fibrils and microcrystals.The microcrystals enabled us to determine the atomic structure of G6V, which proved to be a standard class 2 steric zipper, essentially an amyloid-like protofilament.
The entire 11-residue segment KVKVLGDVIEV forms a <scene name='77/771966/K11v_in_black/2'>hairpin loop</scene> in the 3D structure of ABC.The six residue segment GDVIEV ,termed <scene name='77/771966/Gdviev_black/1'>G6V</scene>,forms fibrils and microcrystals.The microcrystals enabled us to determine the atomic structure of G6V, which proved to be a standard class 2 steric zipper, essentially an amyloid-like protofilament.


Amyloid fibrils and oligomer are both formed by the hairpin segment KVKVLGDVIEV.K11V forms fibrils similar to those of protein(ABC) on shaking at elevated temperature and also similar to K11V^V2L(K11V-TR).The fibrils diameter range from 20 to 100 nm in electrom microscope.G6V,K11V,K11V-TR are all convertible to amyloid state,as is their parent protein ABC.Segment K11V,K11V-TR,and a sequence variant with Leu replacing Val at position 2(K11V^2L) forms stable oligomers intermediate in size between monomer and fiber.
Amyloid fibrils and oligomer are both formed by the hairpin segment KVKVLGDVIEV.K11V forms fibrils similar to those of protein(ABC) on shaking at elevated temperature and also similar to K11V^V2L(K11V-TR).The fibrils diameter range from 20 to 100 nm in electrom microscope.G6V,K11V,K11V-TR are all convertible to amyloid state,as is their parent protein ABC.Segment K11V,K11V-TR,and a sequence variant with Leu replacing Val at position 2(K11V^2L) forms stable oligomers intermediate in size between monomer and fiber.
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