2abw: Difference between revisions
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'''Glutaminase subunit of the plasmodial PLP synthase (Vitamin B6 biosynthesis)''' | '''Glutaminase subunit of the plasmodial PLP synthase (Vitamin B6 biosynthesis)''' | ||
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[[Category: Sinning, I.]] | [[Category: Sinning, I.]] | ||
[[Category: Tews, I.]] | [[Category: Tews, I.]] | ||
[[Category: | [[Category: Glutaminase]] | ||
[[Category: | [[Category: Malaria]] | ||
[[Category: | [[Category: Plp-synthase]] | ||
[[Category: | [[Category: Vitamin b6]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 18:51:38 2008'' | |||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | |||
Revision as of 15:51, 3 May 2008
Glutaminase subunit of the plasmodial PLP synthase (Vitamin B6 biosynthesis)
Overview
Vitamin B6 is one of nature's most versatile cofactors. Most organisms synthesize vitamin B6 via a recently discovered pathway employing the proteins Pdx1 and Pdx2. Here we present an in-depth characterization of the respective orthologs from the malaria parasite, Plasmodium falciparum. Expression profiling of Pdx1 and -2 shows that blood-stage parasites indeed possess a functional vitamin B6 de novo biosynthesis. Recombinant Pdx1 and Pdx2 form a complex that functions as a glutamine amidotransferase with Pdx2 as the glutaminase and Pdx1 as pyridoxal-5 '-phosphate synthase domain. Complex formation is required for catalytic activity of either domain. Pdx1 forms a chimeric bi-enzyme with the bacterial YaaE, a Pdx2 ortholog, both in vivo and in vitro, although this chimera does not attain full catalytic activity, emphasizing that species-specific structural features govern the interaction between the protein partners of the PLP synthase complexes in different organisms. To gain insight into the activation mechanism of the parasite bi-enzyme complex, the three-dimensional structure of Pdx2 was determined at 1.62 A. The obstruction of the oxyanion hole indicates that Pdx2 is in a resting state and that activation occurs upon Pdx1-Pdx2 complex formation.
About this Structure
2ABW is a Single protein structure of sequence from Plasmodium falciparum. Full crystallographic information is available from OCA.
Reference
Vitamin B6 biosynthesis by the malaria parasite Plasmodium falciparum: biochemical and structural insights., Gengenbacher M, Fitzpatrick TB, Raschle T, Flicker K, Sinning I, Muller S, Macheroux P, Tews I, Kappes B, J Biol Chem. 2006 Feb 10;281(6):3633-41. Epub 2005 Dec 8. PMID:16339145 Page seeded by OCA on Sat May 3 18:51:38 2008
