Sandbox k11v: Difference between revisions
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K11V and K11V<sup>V2L</sup> form hexameric oligomers.K11V oligomer is of 6 chains and K11V-TR oligomer of three tandem chains.Other than the glycine linkers and the Val-to-Leu replacement, the cylindrical bodies of the six stranded K11V and the three double stranded K11V-TR oligomers are essentially identical. | K11V and K11V<sup>V2L</sup> form hexameric oligomers.K11V oligomer is of 6 chains and K11V-TR oligomer of three tandem chains.Other than the glycine linkers and the Val-to-Leu replacement, the cylindrical bodies of the six stranded K11V and the three double stranded K11V-TR oligomers are essentially identical. | ||
The structure of K11V is a six-stranded antiparallel barrel of cylindrical in shape also called as cylindrin(Fig. 3).It is completely different in structure from either the native structure of ABC or from G6V segment.Each strand of cylindrin is bonded to one neighbouring strand by a strong interface and to a second by a weak interface.The weak interface is formed by eight hydrogen bonds: four from the main chain, two mediated through side-chain interactions, and two through a water bridge.The strong interface is formed by 12 hydrogen bonds and spreads outward at the ends. | The structure of K11V is a six-stranded antiparallel barrel of cylindrical in shape also called as cylindrin(Fig. 3 & Fig.4).It is completely different in structure from either the native structure of ABC or from G6V segment.Each strand of cylindrin is bonded to one neighbouring strand by a strong interface and to a second by a weak interface.The weak interface is formed by eight hydrogen bonds: four from the main chain, two mediated through side-chain interactions, and two through a water bridge.The strong interface is formed by 12 hydrogen bonds and spreads outward at the ends. | ||
[[Image:Ribbonrepresentationofcrystalcylindrinfig2b.jpg | thumb | 400px | left | Fig. 3 Ribbon representation of the cylindrin crystal structure. ]] | [[Image:Ribbonrepresentationofcrystalcylindrinfig2b.jpg | thumb | 400px | left | Fig. 3 Ribbon representation of the cylindrin crystal structure. ]] | ||
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The structure of K11V-TR was determined, even though glycine linkers and Val-to-Leu replacement,cylindrical bodies of three double stranded K11V-TR oligomers and six-stranded K11V were identical. | The structure of K11V-TR was determined, even though glycine linkers and Val-to-Leu replacement,cylindrical bodies of three double stranded K11V-TR oligomers and six-stranded K11V were identical. | ||
