4lm8: Difference between revisions

Revision as of 11:10, 14 December 2022

Crystal structure of the outer membrane decaheme cytochrome MtrC

Structural highlights

4lm8 is a 1 chain structure with sequence from Shewanella oneidensis MR-1. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	ACT, CA, EDO, HEC
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

Q8EG34_SHEON

Publication Abstract from PubMed

Extracellular microbe-mineral electron transfer is a major driving force for the oxidation of organic carbon in many subsurface environments. Extracellular multi-heme cytochromes of the Shewenella genus play a major role in this process but the mechanism of electron exchange at the interface between cytochrome and acceptor is widely debated. The 1.8 A x-ray crystal structure of the decaheme MtrC revealed a highly conserved CX8C disulfide that, when substituted for AX8A, severely compromised the ability of S. oneidensis to grow under aerobic conditions. Reductive cleavage of the disulfide in the presence of flavin mononucleotide (FMN) resulted in the reversible formation of a stable flavocytochrome. Similar results were also observed with other decaheme cytochromes, OmcA, MtrF and UndA. The data suggest that these decaheme cytochromes can transition between highly reactive flavocytochromes or less reactive cytochromes, and that this transition is controlled by a redox active disulfide that responds to the presence of oxygen.

Redox Linked Flavin Sites in Extracellular Decaheme Proteins Involved in Microbe-Mineral Electron Transfer.,Edwards MJ, White GF, Norman M, Tome-Fernandez A, Ainsworth E, Shi L, Fredrickson JK, Zachara JM, Butt JN, Richardson DJ, Clarke TA Sci Rep. 2015 Jul 1;5:11677. doi: 10.1038/srep11677. PMID:26126857^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Edwards MJ, White GF, Norman M, Tome-Fernandez A, Ainsworth E, Shi L, Fredrickson JK, Zachara JM, Butt JN, Richardson DJ, Clarke TA. Redox Linked Flavin Sites in Extracellular Decaheme Proteins Involved in Microbe-Mineral Electron Transfer. Sci Rep. 2015 Jul 1;5:11677. doi: 10.1038/srep11677. PMID:26126857 doi:https://dx.doi.org/10.1038/srep11677

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OCA

[1] Edwards MJ, White GF, Norman M, Tome-Fernandez A, Ainsworth E, Shi L, Fredrickson JK, Zachara JM, Butt JN, Richardson DJ, Clarke TA. Redox Linked Flavin Sites in Extracellular Decaheme Proteins Involved in Microbe-Mineral Electron Transfer. Sci Rep. 2015 Jul 1;5:11677. doi: 10.1038/srep11677. PMID:26126857 doi:https://dx.doi.org/10.1038/srep11677

[1]

@@ Line 1: / Line 1: @@
 ==Crystal structure of the outer membrane decaheme cytochrome MtrC==
-<StructureSection load='4lm8' size='340' side='right' caption='[[4lm8]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
+<StructureSection load='4lm8' size='340' side='right'caption='[[4lm8]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[4lm8]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Sheon Sheon]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4LM8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4LM8 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[4lm8]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Shewanella_oneidensis_MR-1 Shewanella oneidensis MR-1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4LM8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4LM8 FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=HEC:HEME+C'>HEC</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=HEC:HEME+C'>HEC</scene></td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3pmq|3pmq]], [[3ucp|3ucp]], [[4lmh|4lmh]]</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4lm8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4lm8 OCA], [https://pdbe.org/4lm8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4lm8 RCSB], [https://www.ebi.ac.uk/pdbsum/4lm8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4lm8 ProSAT]</span></td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">mtrC, SO_1778 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=211586 SHEON])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4lm8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4lm8 OCA], [http://pdbe.org/4lm8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4lm8 RCSB], [http://www.ebi.ac.uk/pdbsum/4lm8 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4lm8 ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/Q8EG34_SHEON Q8EG34_SHEON]
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 22: / Line 22: @@
 __TOC__
 </StructureSection>
-[[Category: Sheon]]
+[[Category: Large Structures]]
-[[Category: Clarke, T A]]
+[[Category: Shewanella oneidensis MR-1]]
-[[Category: Edwards, M J]]
+[[Category: Clarke TA]]
-[[Category: C type cytochrome electron transport]]
+[[Category: Edwards MJ]]
-[[Category: Electron transport]]
-[[Category: Greek key]]
-[[Category: Outer membrane]]

4lm8: Difference between revisions

Revision as of 11:10, 14 December 2022

Crystal structure of the outer membrane decaheme cytochrome MtrC

Structural highlights

Function

Publication Abstract from PubMed

References

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