2ard: Difference between revisions

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[[Image:2ard.gif|left|200px]]
[[Image:2ard.gif|left|200px]]


{{Structure
<!--
|PDB= 2ard |SIZE=350|CAPTION= <scene name='initialview01'>2ard</scene>, resolution 2.600&Aring;
The line below this paragraph, containing "STRUCTURE_2ard", creates the "Structure Box" on the page.
|SITE=
You may change the PDB parameter (which sets the PDB file loaded into the applet)
|LIGAND= <scene name='pdbligand=FDA:DIHYDROFLAVINE-ADENINE+DINUCLEOTIDE'>FDA</scene>
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
|ACTIVITY=
or leave the SCENE parameter empty for the default display.
|GENE= prnA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=294 Pseudomonas fluorescens])
-->
|DOMAIN=
{{STRUCTURE_2ard|  PDB=2ard |  SCENE= }}  
|RELATEDENTRY=[[2apg|2APG]], [[2aqj|2AQJ]], [[2ar8|2AR8]]
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ard FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ard OCA], [http://www.ebi.ac.uk/pdbsum/2ard PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2ard RCSB]</span>
}}


'''The structure of tryptophan 7-halogenase (PrnA) suggests a mechanism for regioselective chlorination'''
'''The structure of tryptophan 7-halogenase (PrnA) suggests a mechanism for regioselective chlorination'''
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[[Category: SSPF, Scottish Structural Proteomics Facility.]]
[[Category: SSPF, Scottish Structural Proteomics Facility.]]
[[Category: Unversucht, S.]]
[[Category: Unversucht, S.]]
[[Category: helical bundle]]
[[Category: Helical bundle]]
[[Category: sandwiched sheet]]
[[Category: Sandwiched sheet]]
[[Category: scottish structural proteomics facility]]
[[Category: Scottish structural proteomics facility]]
[[Category: sspf]]
[[Category: Sspf]]
[[Category: structural genomic]]
[[Category: Structural genomic]]
[[Category: tryptophan 7-halogenase,flavin-dependent halogenase]]
[[Category: Tryptophan 7-halogenase,flavin-dependent halogenase]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 19:22:51 2008''
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 01:56:15 2008''

Revision as of 16:22, 3 May 2008

File:2ard.gif

Template:STRUCTURE 2ard

The structure of tryptophan 7-halogenase (PrnA) suggests a mechanism for regioselective chlorination


Overview

Chlorinated natural products include vancomycin and cryptophycin A. Their biosynthesis involves regioselective chlorination by flavin-dependent halogenases. We report the structural characterization of tryptophan 7-halogenase (PrnA), which regioselectively chlorinates tryptophan. Tryptophan and flavin adenine dinucleotide (FAD) are separated by a 10 angstrom-long tunnel and bound by distinct enzyme modules. The FAD module is conserved in halogenases and is related to flavin-dependent monooxygenases. On the basis of biochemical studies, crystal structures, and by analogy with monooxygenases, we predict that FADH2 reacts with O2 to make peroxyflavin, which is decomposed by Cl-. The resulting HOCl is guided through the tunnel to tryptophan, where it is activated to participate in electrophilic aromatic substitution.

About this Structure

2ARD is a Single protein structure of sequence from Pseudomonas fluorescens. Full crystallographic information is available from OCA.

Reference

Tryptophan 7-halogenase (PrnA) structure suggests a mechanism for regioselective chlorination., Dong C, Flecks S, Unversucht S, Haupt C, van Pee KH, Naismith JH, Science. 2005 Sep 30;309(5744):2216-9. PMID:16195462 Page seeded by OCA on Sat May 3 19:22:51 2008

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