5xei

Crystal structure of the Smc head domain with a coiled coil and joint derived from Pyrococcus yayanosii

spinqualitylabelsall models 5xei, resolution 2.60Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 5xei is a 1 chain structure with sequence from Pyrococcus yayanosii CH1. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.599Å Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function F8AFS8_PYRYC Required for chromosome condensation and partitioning.[HAMAP-Rule:MF_01894] Publication Abstract from PubMed Multi-subunit SMC complexes control chromosome superstructure and promote chromosome disjunction, conceivably by actively translocating along DNA double helices. SMC subunits comprise an ABC ATPase "head" and a "hinge" dimerization domain connected by a 49 nm coiled-coil "arm." The heads undergo ATP-dependent engagement and disengagement to drive SMC action on the chromosome. Here, we elucidate the architecture of prokaryotic Smc dimers by high-throughput cysteine cross-linking and crystallography. Co-alignment of the Smc arms tightly closes the interarm space and misaligns the Smc head domains at the end of the rod by close apposition of their ABC signature motifs. Sandwiching of ATP molecules between Smc heads requires them to substantially tilt and translate relative to each other, thereby opening up the Smc arms. We show that this mechanochemical gating reaction regulates chromosome targeting and propose a mechanism for DNA translocation based on the merging of DNA loops upon closure of Smc arms. Structure of Full-Length SMC and Rearrangements Required for Chromosome Organization.,Diebold-Durand ML, Lee H, Ruiz Avila LB, Noh H, Shin HC, Im H, Bock FP, Burmann F, Durand A, Basfeld A, Ham S, Basquin J, Oh BH, Gruber S Mol Cell. 2017 Jul 20;67(2):334-347.e5. doi: 10.1016/j.molcel.2017.06.010. Epub, 2017 Jul 6. PMID:28689660 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Condensin

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