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==Crystal structure of the P228A variant of Thermotoga maritima acetyl esterase==
==Crystal structure of the P228A variant of Thermotoga maritima acetyl esterase==
<StructureSection load='5gma' size='340' side='right' caption='[[5gma]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
<StructureSection load='5gma' size='340' side='right'caption='[[5gma]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[5gma]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Thema Thema]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5GMA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5GMA FirstGlance]. <br>
<table><tr><td colspan='2'>[[5gma]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermotoga_maritima_MSB8 Thermotoga maritima MSB8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5GMA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5GMA FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5fdf|5fdf]], [[5jib|5jib]], [[5hfn|5hfn]]</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene></td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">axeA, TM_0077 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=243274 THEMA])</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5gma FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5gma OCA], [https://pdbe.org/5gma PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5gma RCSB], [https://www.ebi.ac.uk/pdbsum/5gma PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5gma ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5gma FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5gma OCA], [http://pdbe.org/5gma PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5gma RCSB], [http://www.ebi.ac.uk/pdbsum/5gma PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5gma ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[[http://www.uniprot.org/uniprot/CAH_THEMA CAH_THEMA]] Esterase that removes acetyl groups from a number of O-acetylated small substrates, such as acetylated xylose, short xylo-oligosaccharides and cephalosporin C. Has no activity towards polymeric acetylated xylan, 4-methylumbelliferyl acetate or alpha-naphthyl acetate. Able to catalyze rapid hydrolysis of a range of substrates preferably with acetate groups, independent of the alcohol moiety. Exhibits a narrow selectivity for short chain acyl esters (C2-C3). Displays broad substrate specificity by hydrolyzing acetate at 2, 3, and 4 positions of 4-nitrophenyl-beta-D-xylopyranoside (pNP-Xyl) with similar efficiency. Cannot cleave amide linkages.<ref>PMID:21255309</ref> <ref>PMID:22411095</ref> <ref>PMID:22659119</ref>
[https://www.uniprot.org/uniprot/CAH_THEMA CAH_THEMA] Esterase that removes acetyl groups from a number of O-acetylated small substrates, such as acetylated xylose, short xylo-oligosaccharides and cephalosporin C. Has no activity towards polymeric acetylated xylan, 4-methylumbelliferyl acetate or alpha-naphthyl acetate. Able to catalyze rapid hydrolysis of a range of substrates preferably with acetate groups, independent of the alcohol moiety. Exhibits a narrow selectivity for short chain acyl esters (C2-C3). Displays broad substrate specificity by hydrolyzing acetate at 2, 3, and 4 positions of 4-nitrophenyl-beta-D-xylopyranoside (pNP-Xyl) with similar efficiency. Cannot cleave amide linkages.<ref>PMID:21255309</ref> <ref>PMID:22411095</ref> <ref>PMID:22659119</ref>  
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== Publication Abstract from PubMed ==
== Publication Abstract from PubMed ==
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Thema]]
[[Category: Large Structures]]
[[Category: Manoj, N]]
[[Category: Thermotoga maritima MSB8]]
[[Category: Carbohydrate metabolism]]
[[Category: Manoj N]]
[[Category: Cephalosporin deacetylase]]
[[Category: Hydrolase]]
[[Category: Rossmann fold]]
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