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==Crystal structure of eVP30 C-terminus and eNP peptide==
==Crystal structure of eVP30 C-terminus and eNP peptide==
<StructureSection load='5vao' size='340' side='right' caption='[[5vao]], [[Resolution|resolution]] 2.56&Aring;' scene=''>
<StructureSection load='5vao' size='340' side='right'caption='[[5vao]], [[Resolution|resolution]] 2.56&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[5vao]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Ebov Ebov]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5VAO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5VAO FirstGlance]. <br>
<table><tr><td colspan='2'>[[5vao]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Ebola_virus Ebola virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5VAO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5VAO FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.56&#8491;</td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5vap|5vap]]</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">VP30 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1570291 EBOV])</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5vao FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5vao OCA], [https://pdbe.org/5vao PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5vao RCSB], [https://www.ebi.ac.uk/pdbsum/5vao PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5vao ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5vao FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5vao OCA], [http://pdbe.org/5vao PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5vao RCSB], [http://www.ebi.ac.uk/pdbsum/5vao PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5vao ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[[http://www.uniprot.org/uniprot/VP30_EBOZ5 VP30_EBOZ5]] Acts as a transcription anti-termination factor immediately after transcription initiation, but does not affect transcription elongation. This function has been found to be dependent on the formation of an RNA stem-loop at the transcription start site of the first gene. Binds to RNA (By similarity).
[https://www.uniprot.org/uniprot/VP30_EBOZ5 VP30_EBOZ5] Acts as a transcription anti-termination factor immediately after transcription initiation, but does not affect transcription elongation. This function has been found to be dependent on the formation of an RNA stem-loop at the transcription start site of the first gene. Binds to RNA (By similarity).
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Ebola virus (EBOV) is an enveloped negative-sense RNA virus that causes sporadic outbreaks with high case fatality rates. Ebola viral protein 30 (eVP30) plays a critical role in EBOV transcription initiation at the nucleoprotein (eNP) gene, with additional roles in the replication cycle such as viral assembly. However, the mechanistic basis for how eVP30 functions during the virus replication cycle is currently unclear. Here we define a key interaction between eVP30 and a peptide derived from eNP that is important to facilitate interactions leading to the recognition of the RNA template. We present crystal structures of the eVP30 C-terminus in complex with this eNP peptide. Functional analyses of the eVP30-eNP interface identify residues that are critical for viral RNA synthesis. Altogether, these results support a model where the eVP30-eNP interaction plays a critical role in transcription initiation and provides a novel target for the development of antiviral therapy.


Ebola virus VP30 and nucleoprotein interactions modulate viral RNA synthesis.,Xu W, Luthra P, Wu C, Batra J, Leung DW, Basler CF, Amarasinghe GK Nat Commun. 2017 Jun 8;8:15576. doi: 10.1038/ncomms15576. PMID:28593988<ref>PMID:28593988</ref>
==See Also==
 
*[[Nucleoprotein 3D structures|Nucleoprotein 3D structures]]
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 5vao" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Ebov]]
[[Category: Ebola virus]]
[[Category: Amarasinghe, G K]]
[[Category: Large Structures]]
[[Category: Leung, D W]]
[[Category: Amarasinghe GK]]
[[Category: XU, W]]
[[Category: Leung DW]]
[[Category: Functional class]]
[[Category: XU W]]
[[Category: Known biologyical activity]]
[[Category: Viral protein]]
Retrieved from "https://proteopedia.org/5vao"