4tqu: Difference between revisions

Revision as of 15:56, 29 January 2020

Crystal structure of a bacterial ABC transporter involved in the import of the acidic polysaccharide alginate

Structural highlights

4tqu is a 5 chain structure with sequence from Rhizomonas sp.. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	BEM, CA, MAW
Related:	4tqv
Gene:	algM1 (Rhizomonas sp.), algM2 (Rhizomonas sp.), algS (Rhizomonas sp.), algQ2 (Rhizomonas sp.)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Publication Abstract from PubMed

The acidic polysaccharide alginate represents a promising marine biomass for the microbial production of biofuels, although the molecular and structural characteristics of alginate transporters remain to be clarified. In Sphingomonas sp. A1, the ATP-binding cassette transporter AlgM1M2SS is responsible for the import of alginate across the cytoplasmic membrane. Here, we present the substrate-transport characteristics and quaternary structure of AlgM1M2SS. The addition of poly- or oligoalginate enhanced the ATPase activity of reconstituted AlgM1M2SS coupled with one of the periplasmic solute-binding proteins, AlgQ1 or AlgQ2. External fluorescence-labeled oligoalginates were specifically imported into AlgM1M2SS-containing proteoliposomes in the presence of AlgQ2, ATP, and Mg2+. The crystal structure of AlgQ2-bound AlgM1M2SS adopts an inward-facing conformation. The interaction between AlgQ2 and AlgM1M2SS induces the formation of an alginate-binding tunnel-like structure accessible to the solvent. The translocation route inside the transmembrane domains contains charged residues suitable for the import of acidic saccharides.

Structure of a Bacterial ABC Transporter Involved in the Import of an Acidic Polysaccharide Alginate.,Maruyama Y, Itoh T, Kaneko A, Nishitani Y, Mikami B, Hashimoto W, Murata K Structure. 2015 Jul 21. pii: S0969-2126(15)00274-9. doi:, 10.1016/j.str.2015.06.021. PMID:26235029^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Maruyama Y, Itoh T, Kaneko A, Nishitani Y, Mikami B, Hashimoto W, Murata K. Structure of a Bacterial ABC Transporter Involved in the Import of an Acidic Polysaccharide Alginate. Structure. 2015 Jul 21. pii: S0969-2126(15)00274-9. doi:, 10.1016/j.str.2015.06.021. PMID:26235029 doi:https://dx.doi.org/10.1016/j.str.2015.06.021

Proteopedia Page Contributors and Editors (what is this?)

OCA

[1] Maruyama Y, Itoh T, Kaneko A, Nishitani Y, Mikami B, Hashimoto W, Murata K. Structure of a Bacterial ABC Transporter Involved in the Import of an Acidic Polysaccharide Alginate. Structure. 2015 Jul 21. pii: S0969-2126(15)00274-9. doi:, 10.1016/j.str.2015.06.021. PMID:26235029 doi:https://dx.doi.org/10.1016/j.str.2015.06.021

[1]

@@ Line 1: / Line 1: @@
 ==Crystal structure of a bacterial ABC transporter involved in the import of the acidic polysaccharide alginate==
-<StructureSection load='4tqu' size='340' side='right' caption='[[4tqu]], [[Resolution|resolution]] 3.20&Aring;' scene=''>
+<StructureSection load='4tqu' size='340' side='right'caption='[[4tqu]], [[Resolution|resolution]] 3.20&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[4tqu]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/'rhizomonas'_sp. 'rhizomonas' sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4TQU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4TQU FirstGlance]. <br>
+<table><tr><td colspan='2'>[[4tqu]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/Rhizomonas_sp. Rhizomonas sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4TQU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4TQU FirstGlance]. <br>
 </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BEM:BETA-D-MANNURONIC+ACID'>BEM</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MAW:4-DEOXY-ALPHA-L-ERYTHRO-HEX-4-ENOPYRANURONIC+ACID'>MAW</scene></td></tr>
 <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4tqv|4tqv]]</td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">algM1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=28214 'Rhizomonas' sp.]), algM2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=28214 'Rhizomonas' sp.]), algS ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=28214 'Rhizomonas' sp.]), algQ2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=28214 'Rhizomonas' sp.])</td></tr>
+<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">algM1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=28214 Rhizomonas sp.]), algM2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=28214 Rhizomonas sp.]), algS ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=28214 Rhizomonas sp.]), algQ2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=28214 Rhizomonas sp.])</td></tr>
 <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4tqu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4tqu OCA], [http://pdbe.org/4tqu PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4tqu RCSB], [http://www.ebi.ac.uk/pdbsum/4tqu PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4tqu ProSAT]</span></td></tr>
 </table>
@@ Line 22: / Line 22: @@
 __TOC__
 </StructureSection>
+[[Category: Large Structures]]
 [[Category: Rhizomonas sp]]
 [[Category: Hashimoto, W]]

4tqu: Difference between revisions

Revision as of 15:56, 29 January 2020

Crystal structure of a bacterial ABC transporter involved in the import of the acidic polysaccharide alginate

Structural highlights

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Navigation menu

Page actions

Page actions

Personal tools

Navigation

Search

Tools