4b5n

Crystal structure of oxidized Shewanella Yellow Enzyme 4 (SYE4)

spinqualitylabelsall models 4b5n, resolution 1.10Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 4b5n is a 1 chain structure with sequence from Sheon. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: 8K6, FMN Activity: NADPH dehydrogenase, with EC number 1.6.99.1 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed Shewanella oneidensis, a Gram-negative gamma-proteobacterium with an extensive redox capacity, possesses four old yellow enzyme (OYE) homologs. Of these, Shewanella yellow enzyme 4 (SYE4) is implicated in resistance to oxidative stress. Here, we present a series of high-resolution crystal structures for SYE4 in the oxidized and reduced states, and in complex with phenolic ligands and the nitro-aromatic explosive picric acid. The structures unmask new features, including the identification of a binding platform for long-chain hydrophobic molecules. Furthermore, we present the first structural observation of a hydride-Meisenheimer complex of picric acid with a flavoenzyme. Overall, our study exposes the binding promiscuity of SYE4 toward a variety of electrophilic substrates and is consistent with a general detoxification function for SYE4. Structural dissection of Shewanella oneidensis old yellow enzyme 4 bound to a Meisenheimer complex and (nitro)phenolic ligands.,Elegheert J, Brige A, Van Beeumen J, Savvides SN FEBS Lett. 2017 Sep 4. doi: 10.1002/1873-3468.12833. PMID:28869767 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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