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'''CATALYTIC DOMAIN OF ENDO-1,4-GLUCANASE CEL6A MUTANT Y73S FROM THERMOBIFIDA FUSCA''' | '''CATALYTIC DOMAIN OF ENDO-1,4-GLUCANASE CEL6A MUTANT Y73S FROM THERMOBIFIDA FUSCA''' | ||
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[[Category: Roos, A.]] | [[Category: Roos, A.]] | ||
[[Category: Wilson, D B.]] | [[Category: Wilson, D B.]] | ||
[[Category: | [[Category: Carbohydrate metabolism]] | ||
[[Category: | [[Category: Cellulose degradation]] | ||
[[Category: | [[Category: Endoglucanase]] | ||
[[Category: | [[Category: Glycosidase]] | ||
[[Category: | [[Category: Glycoside hydrolase family 6]] | ||
[[Category: | [[Category: Hydrolase]] | ||
[[Category: | [[Category: Methyl cellobiosyl-4-thio-beta-cellobioside]] | ||
[[Category: | [[Category: Polysaccharide degradation]] | ||
[[Category: | [[Category: Thermobifida fusca]] | ||
[[Category: | [[Category: Tim a/b fold]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 20:34:20 2008'' | |||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | |||
Revision as of 17:34, 3 May 2008
CATALYTIC DOMAIN OF ENDO-1,4-GLUCANASE CEL6A MUTANT Y73S FROM THERMOBIFIDA FUSCA
Overview
Endoglucanase Cel6A from Thermobifida fusca hydrolyzes the beta-1,4 linkages in cellulose at accessible points along the polymer. The structure of the catalytic domain of Cel6A from T. fusca in complex with a nonhydrolysable substrate analogue that acts as an inhibitor, methylcellobiosyl-4-thio-beta-cellobioside (Glc(2)-S-Glc(2)), has been determined to 1.5 A resolution. The glycosyl unit in subsite -1 was sterically hindered by Tyr73 and forced into a distorted (2)S(o) conformation. In the enzyme where Tyr73 was mutated to a serine residue, the hindrance was removed and the glycosyl unit in subsite -1 had a relaxed (4)C(1) chair conformation. The relaxed conformation was seen in two complex structures of the mutated enzyme, with cellotetrose (Glc(4)) at 1.64 A and Glc(2)-S-Glc(2) at 1.04 A resolution.
About this Structure
2BOE is a Single protein structure of sequence from Thermobifida fusca. Full crystallographic information is available from OCA.
Reference
Crystal structure of Thermobifida fusca endoglucanase Cel6A in complex with substrate and inhibitor: the role of tyrosine Y73 in substrate ring distortion., Larsson AM, Bergfors T, Dultz E, Irwin DC, Roos A, Driguez H, Wilson DB, Jones TA, Biochemistry. 2005 Oct 4;44(39):12915-22. PMID:16185060 Page seeded by OCA on Sat May 3 20:34:20 2008
Proteopedia Page Contributors and Editors (what is this?)
- Pages with broken file links
- Cellulase
- Single protein
- Thermobifida fusca
- Bergfors, T.
- Driguez, H.
- Dultz, E.
- Irwin, D C.
- Jones, T A.
- Larsson, A M.
- Roos, A.
- Wilson, D B.
- Carbohydrate metabolism
- Cellulose degradation
- Endoglucanase
- Glycosidase
- Glycoside hydrolase family 6
- Hydrolase
- Methyl cellobiosyl-4-thio-beta-cellobioside
- Polysaccharide degradation
- Tim a/b fold
