Unusual sequence numbering: Difference between revisions
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'''Negative.''' Sometimes the initial sequence number is negative. This is usually done when residues were engineered onto the N-terminus. The transition from -1 to 1 may or may not include a residue numbered zero. An example is [http://firstglance.jmol.org/fg.htm?mol=1d5t 1d5t] ([[1d5t]]). The N-terminal Met of the [http://www.uniprot.org/uniprot/P21856#sequences genomic sequence] is numbered 1. But a di-histidine tag was engineered onto the N-terminus: His -2, His -1, Met 1. In this case, there is no residue numbered zero. The C-terminal residue is Phe431, but the length of the genomic sequence is 447. The C-terminal 16 residues of the genomic sequence were not present in the crystallized protein. In this model, no residues are missing due to crystallographic disorder. | '''Negative.''' Sometimes the initial sequence number is negative. This is usually done when residues were engineered onto the N-terminus. The transition from -1 to 1 may or may not include a residue numbered zero. An example is [http://firstglance.jmol.org/fg.htm?mol=1d5t 1d5t] ([[1d5t]]). The N-terminal Met of the [http://www.uniprot.org/uniprot/P21856#sequences genomic sequence] is numbered 1. But a di-histidine tag was engineered onto the N-terminus: His -2, His -1, Met 1. In this case, there is no residue numbered zero. The C-terminal residue is Phe431, but the length of the genomic sequence is 447. The C-terminal 16 residues of the genomic sequence were not present in the crystallized protein. In this model, no residues are missing due to crystallographic disorder. | ||
Another example is [http://firstglance.jmol.org/fg.htm?mol=4ifd 4ifd] ([[4ifd]]), where chain R includes RNA residues numbered -1 to - | Another example is [http://firstglance.jmol.org/fg.htm?mol=4ifd 4ifd] ([[4ifd]]), where chain R includes RNA residues numbered -1 to -15 and -30 to -44. The 5' end is numbered -44 and the 3' end, -1. | ||
==Multiple Residues with the Same Number== | ==Multiple Residues with the Same Number== | ||
