6bqw: Difference between revisions

Line 1:

~~'''Unreleased structure'''~~

~~The entry~~ 6bqw is ~~ON HOLD~~

==AlfA Filament bound to AMPPNP==

== Structural highlights ==

<table><tr><td colspan='2'>[[6bqw]] is a 9 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6BQW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6BQW FirstGlance]. <br>

</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ANP:PHOSPHOAMINOPHOSPHONIC+ACID-ADENYLATE+ESTER'>ANP</scene></td></tr>

<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6bqw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6bqw OCA], [http://pdbe.org/6bqw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6bqw RCSB], [http://www.ebi.ac.uk/pdbsum/6bqw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6bqw ProSAT]</span></td></tr>

</table>

== Publication Abstract from PubMed ==

Bacterial actins are an evolutionarily diverse family of ATP-dependent filaments built from protomers with a conserved structural fold. Actin-based segregation systems are encoded on many bacterial plasmids and function to partition plasmids into daughter cells. The bacterial actin AlfA segregates plasmids by a mechanism distinct from other partition systems, dependent on its unique dynamic properties. Here, we report the near-atomic resolution electron cryo-microscopy structure of the AlfA filament, which reveals a strikingly divergent filament architecture resulting from the loss of a subdomain conserved in all other actins and a mode of ATP binding. Its unusual assembly interfaces and nucleotide interactions provide insight into AlfA dynamics, and expand the range of evolutionary variation accessible to actin quaternary structure.

~~Authors:~~ Usluer, G.D., ~~Kollman, J~~.M.~~, DiMaio, F~~.

Cryo-EM structure of the bacterial actin AlfA reveals unique assembly and ATP-binding interactions and the absence of a conserved subdomain.,Usluer GD, DiMaio F, Yang SK, Hansen JM, Polka JK, Mullins RD, Kollman JM Proc Natl Acad Sci U S A. 2018 Feb 13. pii: 1715836115. doi:, 10.1073/pnas.1715836115. PMID:29440491<ref>PMID:29440491</ref>

~~Description~~: ~~AlfA Filament bound to AMPPNP~~

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>

[[Category: ~~Unreleased Structures~~]]

</div>

[[Category: ~~Dimaio~~, F]]

[[Category: Usluer, G.D]]

== References ==

[[Category: ~~Kollman, J.M~~]]

__TOC__

</StructureSection>

[[Category: DiMaio, F]]

[[Category: Kollman, J M]]

[[Category: Usluer, G D]]

[[Category: Actin]]

[[Category: Cytosolic protein]]

[[Category: Filament]]

[[Category: Plasmid segregation]]

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@@ Line 1: / Line 1: @@
-'''Unreleased structure'''
-The entry 6bqw is ON HOLD
+==AlfA Filament bound to AMPPNP==
+<StructureSection load='6bqw' size='340' side='right' caption='[[6bqw]], [[Resolution|resolution]] 4.20&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[6bqw]] is a 9 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6BQW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6BQW FirstGlance]. <br>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ANP:PHOSPHOAMINOPHOSPHONIC+ACID-ADENYLATE+ESTER'>ANP</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6bqw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6bqw OCA], [http://pdbe.org/6bqw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6bqw RCSB], [http://www.ebi.ac.uk/pdbsum/6bqw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6bqw ProSAT]</span></td></tr>
+</table>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Bacterial actins are an evolutionarily diverse family of ATP-dependent filaments built from protomers with a conserved structural fold. Actin-based segregation systems are encoded on many bacterial plasmids and function to partition plasmids into daughter cells. The bacterial actin AlfA segregates plasmids by a mechanism distinct from other partition systems, dependent on its unique dynamic properties. Here, we report the near-atomic resolution electron cryo-microscopy structure of the AlfA filament, which reveals a strikingly divergent filament architecture resulting from the loss of a subdomain conserved in all other actins and a mode of ATP binding. Its unusual assembly interfaces and nucleotide interactions provide insight into AlfA dynamics, and expand the range of evolutionary variation accessible to actin quaternary structure.
-Authors: Usluer, G.D., Kollman, J.M., DiMaio, F.
+Cryo-EM structure of the bacterial actin AlfA reveals unique assembly and ATP-binding interactions and the absence of a conserved subdomain.,Usluer GD, DiMaio F, Yang SK, Hansen JM, Polka JK, Mullins RD, Kollman JM Proc Natl Acad Sci U S A. 2018 Feb 13. pii: 1715836115. doi:, 10.1073/pnas.1715836115. PMID:29440491<ref>PMID:29440491</ref>
-Description: AlfA Filament bound to AMPPNP
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-[[Category: Unreleased Structures]]
+</div>
-[[Category: Dimaio, F]]
+<div class="pdbe-citations 6bqw" style="background-color:#fffaf0;"></div>
-[[Category: Usluer, G.D]]
+== References ==
-[[Category: Kollman, J.M]]
+<references/>
+__TOC__
+</StructureSection>
+[[Category: DiMaio, F]]
+[[Category: Kollman, J M]]
+[[Category: Usluer, G D]]
+[[Category: Actin]]
+[[Category: Cytosolic protein]]
+[[Category: Filament]]
+[[Category: Plasmid segregation]]