1eqp

EXO-B-(1,3)-GLUCANASE FROM CANDIDA ALBICANS

spinqualitylabelsall models 1eqp, resolution 1.90Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 1eqp is a 1 chain structure with sequence from Candida albicans. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Related: 1cz1, 1eqc Activity: Glucan 1,3-beta-glucosidase, with EC number 3.2.1.58 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function [EXG_CANAL] Beta-glucanases participate in the metabolism of beta-glucan, the main structural component of the cell wall. It could also function biosynthetically as a transglycosylase. Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMed In some species of Candida the CUG codon is encoded as serine and not leucine. In the case of the exo-beta-1,3-glucanase from the pathogenic fungus C. albicans there are two such translational events, one in the prepro-leader sequence and the other at residue 64. Overexpression of active mature enzyme in a yeast host indicated that these two positions are tolerant to substitution. By comparing the crystal structure of the recombinant protein with that of the native (presented here), it is seen how either serine or leucine can be accommodated at position 64. Examination of the relatively few solved protein structures from C. albicans indicates that other CUG encoded serines are also found at non-essential surface sites. However such codon usage is rare in C. albicans, in contrast to C. rugosa, with direct implications for respective recombinant protein production. Minor structural consequences of alternative CUG codon usage (Ser for Leu) in Candida albicans exoglucanase.,Cutfield JF, Sullivan PA, Cutfield SM Protein Eng. 2000 Oct;13(10):735-8. PMID:11112513 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Glucanase 3D structures

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