6bp7: Difference between revisions
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==Recombinant major vault protein [Rattus norvegicus] structure in solution: conformation 2== | |||
<StructureSection load='6bp7' size='340' side='right' caption='[[6bp7]], [[Resolution|resolution]] 4.90Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[6bp7]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6BP7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6BP7 FirstGlance]. <br> | |||
</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[6bp8|6bp8]]</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6bp7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6bp7 OCA], [http://pdbe.org/6bp7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6bp7 RCSB], [http://www.ebi.ac.uk/pdbsum/6bp7 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6bp7 ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[[http://www.uniprot.org/uniprot/MVP_RAT MVP_RAT]] Required for normal vault structure. Vaults are multi-subunit structures that may act as scaffolds for proteins involved in signal transduction. Vaults may also play a role in nucleo-cytoplasmic transport. Down-regulates INFG-mediated STAT1 signaling and subsequent activation of JAK. Down-regulates SRC activity and signaling through MAP kinases (By similarity). | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Prior crystal structures of the vault have provided clues of its structural variability but are non-conclusive due to crystal packing. Here, we obtained vaults by engineering at the N terminus of rat major vault protein (MVP) an HIV-1 Gag protein segment and determined their near-atomic resolution ( approximately 4.8 A) structures in a solution/non-crystalline environment. The barrel-shaped vaults in solution adopt two conformations, 1 and 2, both with D39 symmetry. From the N to C termini, each MVP monomer has three regions: body, shoulder, and cap. While conformation 1 is identical to one of the crystal structures, the shoulder in conformation 2 is translocated longitudinally up to 10 A, resulting in an outward-projected cap. Our structures clarify the structural discrepancies in the body region in the prior crystallography models. The vault's drug-delivery potential is highlighted by the internal disposition and structural flexibility of its Gag-loaded N-terminal extension at the barrel waist of the engineered vault. | |||
Solution Structures of Engineered Vault Particles.,Ding K, Zhang X, Mrazek J, Kickhoefer VA, Lai M, Ng HL, Yang OO, Rome LH, Zhou ZH Structure. 2018 Mar 7. pii: S0969-2126(18)30054-6. doi:, 10.1016/j.str.2018.02.014. PMID:29551289<ref>PMID:29551289</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
<div class="pdbe-citations 6bp7" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Ding, K]] | |||
[[Category: Kickhoefer, V A]] | |||
[[Category: Lai, M]] | [[Category: Lai, M]] | ||
[[Category: Mrazek, J]] | [[Category: Mrazek, J]] | ||
[[Category: Rome, L | [[Category: Ng, H L]] | ||
[[Category: Yang, O | [[Category: Rome, L H]] | ||
[[Category: Yang, O O]] | |||
[[Category: Zhang, X]] | [[Category: Zhang, X]] | ||
[[Category: | [[Category: Zhou, Z H]] | ||
[[Category: | [[Category: Structural protein]] | ||
[[Category: | [[Category: Vault recombinant protein structure engineered nano-particle]] | ||
Revision as of 05:57, 4 April 2018
Recombinant major vault protein [Rattus norvegicus] structure in solution: conformation 2
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