2vn7

Glycoside Hydrolase Family 15 Glucoamylase from Hypocrea jecorina

spinqualitylabelsall models 2vn7, resolution 1.90Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 2vn7 is a 1 chain structure with sequence from Hypocrea jecorina. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: BTB, CA, MAN, NAG Related: 2vn4 Activity: Glucan 1,4-alpha-glucosidase, with EC number 3.2.1.3 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMed The three-dimensional structure of a complete Hypocrea jecorina glucoamylase has been determined at 1.8 A resolution. The presented structure model includes the catalytic and starch binding domains and traces the course of the 37-residue linker segment. While the structures of other fungal and yeast glucoamylase catalytic and starch binding domains have been determined separately, this is the first intact structure that allows visualization of the juxtaposition of the starch binding domain relative to the catalytic domain. The detailed interactions we see between the catalytic and starch binding domains are confirmed in a second independent structure determination of the enzyme in a second crystal form. This second structure model exhibits an identical conformation compared to the first structure model, which suggests that the H. jecorina glucoamylase structure we report is independent of crystal lattice contact restraints and represents the three-dimensional structure found in solution. The proposed starch binding regions for the starch binding domain are aligned with the catalytic domain in the three-dimensional structure in a manner that supports the hypothesis that the starch binding domain serves to target the glucoamylase at sites where the starch granular matrix is disrupted and where the enzyme might most effectively function. Three-dimensional structure of an intact glycoside hydrolase family 15 glucoamylase from Hypocrea jecorina.,Bott R, Saldajeno M, Cuevas W, Ward D, Scheffers M, Aehle W, Karkehabadi S, Sandgren M, Hansson H Biochemistry. 2008 May 27;47(21):5746-54. Epub 2008 May 6. PMID:18457422 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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