1iqp

Crystal Structure of the Clamp Loader Small Subunit from Pyrococcus furiosus

spinqualitylabelsall models 1iqp, resolution 2.80Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 1iqp is a 6 chain structure with sequence from Atcc 43587. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: ADP Gene: rfcs (ATCC 43587) Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function [RFCS_PYRFU] Part of the RFC clamp loader complex which loads the PCNA sliding clamp onto DNA. The complex possesses DNA-dependent ATPase activity which is further stimulated by PCNA. Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMed In eukaryotic DNA replication, replication factor-C (RFC) acts as the clamp loader, which correctly installs the sliding clamp onto DNA strands at replication forks. The eukaryotic RFC is a complex consisting of one large and four small subunits. We have determined the crystal structure of the clamp loader small subunit (RFCS) from Pyrococcus furiosus. The six subunits, of which four bind ADP in their canonical nucleotide binding clefts, assemble into a dimer of semicircular trimers. The crescent-like architecture of each subunit formed by the three domains resembles that of the delta' subunit of the E. coli clamp loader. The trimeric architecture of archaeal RFCS, with its mobile N-terminal domains, involves intersubunit interactions that may be conserved in eukaryotic functional complexes. Atomic structure of the clamp loader small subunit from Pyrococcus furiosus.,Oyama T, Ishino Y, Cann IK, Ishino S, Morikawa K Mol Cell. 2001 Aug;8(2):455-63. PMID:11545747 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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