3nk3

Crystal structure of full-length sperm receptor ZP3 at 2.6 A resolution

spinqualitylabelsall models 3nk3, resolution 2.60Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 3nk3 is a 4 chain structure with sequence from Chick. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: A2G, FLC, GAL Related: 3d4c, 3d4g, 3ef7, 3nk4 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function [ZP3_CHICK] Component of the zona pellucida, which mediates species-specific sperm binding. Directly binds to sperm. Important for egg fertilization. Publication Abstract from PubMed ZP3, a major component of the zona pellucida (ZP) matrix coating mammalian eggs, is essential for fertilization by acting as sperm receptor. By retaining a propeptide that contains a polymerization-blocking external hydrophobic patch (EHP), we determined the crystal structure of an avian homolog of ZP3 at 2.0 A resolution. The structure unveils the fold of a complete ZP domain module in a homodimeric arrangement required for secretion and reveals how EHP prevents premature incorporation of ZP3 into the ZP. This suggests mechanisms underlying polymerization and how local structural differences, reflected by alternative disulfide patterns, control the specificity of ZP subunit interaction. Close relative positioning of a conserved O-glycan important for sperm binding and the hypervariable, positively selected C-terminal region of ZP3 suggests a concerted role in the regulation of species-restricted gamete recognition. Alternative conformations of the area around the O-glycan indicate how sperm binding could trigger downstream events via intramolecular signaling. Insights into egg coat assembly and egg-sperm interaction from the X-ray structure of full-length ZP3.,Han L, Monne M, Okumura H, Schwend T, Cherry AL, Flot D, Matsuda T, Jovine L Cell. 2010 Oct 29;143(3):404-15. Epub 2010 Oct 21. PMID:20970175 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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