T7 RNA Polymerase: Difference between revisions
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==T7 RNA Polymerase Initiation Complex== | ==T7 RNA Polymerase Initiation Complex== | ||
<StructureSection load='1qln' size='450' side='right' caption='Promoter bound polymerase' scene=''> | <StructureSection load='1qln' size='450' side='right' caption='Promoter bound polymerase (PDB code [[1qln]])' scene=''> | ||
'''Promoter binding'''. T7 RNA polymerase <scene name='77/778917/T7rp_promo_bound_basic/1'>binds and melts into dsDNA</scene>, by recognizing the upstream duplex region of the promoter (-17 to -5), and then melting a bubble (-4 to about +3), within the larger duplex. The duplex promoter domain binds primarily to the N-terminal domain of the enzyme, with the exception of the (C-terminal domain) "<scene name='77/778917/T7rp_promo_bound_specif_loop_z/2'>specificity loop</scene>." It is the combination of the N-terminal domain, with the positioned specificity loop, that forms the specific binding surface. | '''Promoter binding'''. T7 RNA polymerase <scene name='77/778917/T7rp_promo_bound_basic/1'>binds and melts into dsDNA</scene>, by recognizing the upstream duplex region of the promoter (-17 to -5), and then melting a bubble (-4 to about +3), within the larger duplex. The duplex promoter domain binds primarily to the N-terminal domain of the enzyme, with the exception of the (C-terminal domain) "<scene name='77/778917/T7rp_promo_bound_specif_loop_z/2'>specificity loop</scene>." It is the combination of the N-terminal domain, with the positioned specificity loop, that forms the specific binding surface. | ||
