6c7z: Difference between revisions

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'''Unreleased structure'''


The entry 6c7z is ON HOLD  until Paper Publication
==Crystal structure of the Q108K:K40L:T51V:R58F mutant of human Cellular Retinol Binding Protein II in complex with synthetic Ligand Julolidine==
<StructureSection load='6c7z' size='340' side='right' caption='[[6c7z]], [[Resolution|resolution]] 1.42&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6c7z]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6C7Z OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6C7Z FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=JLO:(2E,4E)-3-methyl-5-(2,3,6,7-tetrahydro-1H,5H-pyrido[3,2,1-ij]quinolin-9-yl)penta-2,4-dienal'>JLO</scene></td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5f58|5f58]]</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6c7z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6c7z OCA], [http://pdbe.org/6c7z PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6c7z RCSB], [http://www.ebi.ac.uk/pdbsum/6c7z PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6c7z ProSAT]</span></td></tr>
</table>
== Function ==
[[http://www.uniprot.org/uniprot/RET2_HUMAN RET2_HUMAN]] Intracellular transport of retinol.
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Mutants of human Cellular Retinol Binding Protein II (hCRBPII) were engineered to bind a julolidine retinal analog for the purpose of developing a ratiometric pH sensor. The design relied on the electrostatic influence of a titratable amino acid side chain, affecting the absorption, and thus emission of the protein/fluorophore complex. The ratio of emissions obtained at two excitation wavelengths that correspond to the absorption of the two forms of the protein/fluorophore complex, leads to a concentration independent measure of pH.


Authors: Nosrati, M., Geiger, J.H.
A Genetically Encoded Ratiometric pH-Probe: Wavelength Regulation-Inspired Design of pH Indicators.,Berbasova T, Tahmasebi Nick S, Nosrati M, Nossoni Z, Santos E, Vasileiou C, Geiger J, Borhan B Chembiochem. 2018 Apr 12. doi: 10.1002/cbic.201800050. PMID:29645331<ref>PMID:29645331</ref>


Description: Crystal structure of the Q108K:K40L:T51V:R58F mutant of human Cellular Retinol Binding Protein II in complex with synthetic Ligand Julolidine
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
[[Category: Unreleased Structures]]
</div>
[[Category: Geiger, J.H]]
<div class="pdbe-citations 6c7z" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Geiger, J H]]
[[Category: Nosrati, M]]
[[Category: Nosrati, M]]
[[Category: Hcrbpii]]
[[Category: Protein engineering]]
[[Category: Ratiometric ph probe]]
[[Category: Transport protein]]
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