5oq1

Crystal structure of Serratia marcescens ChiX (used as MR model for superior PDB 5OPZ)

spinqualitylabelsall models 5oq1, resolution 1.34Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 5oq1 is a 2 chain structure with sequence from Serratia marcescens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 1.34Å Ligands: CL, ZN Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed The Gram-negative bacterium Serratia marcescens secretes a number of proteins that are involved in extracellular chitin degradation. This so-called chitinolytic machinery includes three types of chitinase enzymes and a lytic polysaccharide monooxygenase. An operon has been identified in S. marcescens , chiWXYZ , that is thought to be involved in the secretion of the chitinolytic machinery. Genetic evidence points to the ChiX protein being a key player in the secretion mechanism, since deletion of the chiX gene in S. marcescens led to a mutant strain blocked for secretion of all members of the chitinolytic machinery. In this work, a detailed structural and biochemical characterisation of ChiX is presented. The high resolution crystal structure of ChiX reveals the protein to be a member of the LAS family of peptidases. ChiX is shown to be a Zinc-containing metalloenzyme and in vitro assays demonstrate ChiX is an L-Ala D-Glu endopeptidase that cleaves the crosslinks in bacterial peptidoglycan. This catalytic activity is shown to be intimately linked with the secretion of the chitinolytic machinery, since substitution of the ChiX Asp-120 residue results in a variant protein that is both unable to digest peptidoglycan and cannot rescue the phenoytype of a chiX mutant strain. Structure and activity of ChiX, a peptidoglycan hydrolase required for chitinase secretion by Serratia marcescens .,Owen RA, Fyfe PK, Lodge A, Biboy J, Vollmer W, Hunter WN, Sargent F Biochem J. 2017 Dec 11. pii: BCJ20170633. doi: 10.1042/BCJ20170633. PMID:29229757 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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