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'''Mycobacterium tuberculosis EmbR''' | '''Mycobacterium tuberculosis EmbR''' | ||
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[[Category: Besra, G S.]] | [[Category: Besra, G S.]] | ||
[[Category: Futterer, K.]] | [[Category: Futterer, K.]] | ||
[[Category: | [[Category: Beta-sandwich;]] | ||
[[Category: | [[Category: Tetratricopeptide repeat]] | ||
[[Category: | [[Category: Transcriptional regulator]] | ||
[[Category: | [[Category: Winged-helix]] | ||
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Revision as of 00:49, 4 May 2008
Mycobacterium tuberculosis EmbR
Overview
Ser/Thr phosphorylation has emerged as a critical regulatory mechanism in a number of bacteria, including Mycobacterium tuberculosis. This problematic pathogen encodes 11 eukaryotic-like Ser/Thr kinases, yet few substrates or signaling targets have been characterized. Here, we report the structure of EmbR (2.0 A), a putative transcriptional regulator of key arabinosyltransferases (EmbC, -A, and -B), and an endogenous substrate of the Ser/Thr-kinase PknH. EmbR presents a unique domain architecture: the N-terminal winged-helix DNA-binding domain forms an extensive interface with the all-helical central bacterial transcriptional activation domain and is positioned adjacent to the regulatory C-terminal forkhead-associated (FHA) domain, which mediates binding to a Thr-phosphorylated site in PknH. The structure in complex with a phospho-peptide (1.9 A) reveals a conserved mode of phospho-threonine recognition by the FHA domain and evidence for specific recognition of the cognate kinase. The present structures suggest hypotheses as to how EmbR might propagate the phospho-relay signal from its cognate kinase, while serving as a template for the structurally uncharacterized Streptomyces antibiotic regulatory protein family of transcription factors.
About this Structure
2FEZ is a Single protein structure of sequence from Mycobacterium tuberculosis. Full crystallographic information is available from OCA.
Reference
Molecular structure of EmbR, a response element of Ser/Thr kinase signaling in Mycobacterium tuberculosis., Alderwick LJ, Molle V, Kremer L, Cozzone AJ, Dafforn TR, Besra GS, Futterer K, Proc Natl Acad Sci U S A. 2006 Feb 21;103(8):2558-63. Epub 2006 Feb 13. PMID:16477027 Page seeded by OCA on Sun May 4 03:49:04 2008
