5zfs: Difference between revisions
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==Crystal structure of Arthrobacter globiformis M30 sugar epimerase which can produce D-allulose from D-fructose== | |||
<StructureSection load='5zfs' size='340' side='right' caption='[[5zfs]], [[Resolution|resolution]] 1.96Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[5zfs]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ZFS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ZFS FirstGlance]. <br> | |||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5zfs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5zfs OCA], [http://pdbe.org/5zfs PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5zfs RCSB], [http://www.ebi.ac.uk/pdbsum/5zfs PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5zfs ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The X-ray structure of ketose 3-epimerase from Arthrobacter globiformis M30, which was previously reported to be a D-allulose 3-epimerase (AgD-AE), was determined at 1.96 A resolution. The crystal belonged to the hexagonal space group P6522, with unit-cell parameters a = b = 103.98, c = 256.53 A. The structure was solved by molecular replacement using the structure of Mesorhizobium loti L-ribulose 3-epimerase (MlL-RE), which has 41% sequence identity, as a search model. A hexagonal crystal contained two molecules in the asymmetric unit, and AgD-AE formed a homotetramer with twofold symmetry. The overall structure of AgD-AE was more similar to that of MlL-RE than to the known structures of D-psicose (alternative name D-allulose) 3-epimerases (D-PEs or D-AEs), although AgD-AE and MlL-RE have different substrate specificities. Both AgD-AE and MlL-RE have long helices in the C-terminal region that would contribute to the stability of the homotetramer. AgD-AE showed higher enzymatic activity for L-ribulose than D-allulose; however, AgD-AE is stable and is a unique useful enzyme for the production of D-allulose from D-fructose. | |||
X-ray structure of Arthrobacter globiformis M30 ketose 3-epimerase for the production of D-allulose from D-fructose.,Yoshida H, Yoshihara A, Gullapalli PK, Ohtani K, Akimitsu K, Izumori K, Kamitori S Acta Crystallogr F Struct Biol Commun. 2018 Oct 1;74(Pt 10):669-676. doi:, 10.1107/S2053230X18011706. Epub 2018 Sep 21. PMID:30279320<ref>PMID:30279320</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: | <div class="pdbe-citations 5zfs" style="background-color:#fffaf0;"></div> | ||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Akimitsu, K]] | |||
[[Category: Gullapalli, P K]] | |||
[[Category: Izumori, K]] | |||
[[Category: Kamitori, S]] | |||
[[Category: Ohtani, K]] | [[Category: Ohtani, K]] | ||
[[Category: Yoshida, H]] | [[Category: Yoshida, H]] | ||
[[Category: | [[Category: Yoshihara, A]] | ||
[[Category: | [[Category: Epimerase]] | ||
[[Category: Isomerase]] | |||
[[Category: | |||
Revision as of 05:47, 24 October 2018
Crystal structure of Arthrobacter globiformis M30 sugar epimerase which can produce D-allulose from D-fructose
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