6cq2: Difference between revisions
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==Crystal structure of Mycobacterium tuberculosis Topoisomerase I in complex with oligonucleotide MTS2-12 and Magnesium== | |||
<StructureSection load='6cq2' size='340' side='right' caption='[[6cq2]], [[Resolution|resolution]] 3.00Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[6cq2]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6CQ2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6CQ2 FirstGlance]. <br> | |||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr> | |||
[[Category: | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5d5h|5d5h]]</td></tr> | ||
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA_topoisomerase DNA topoisomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.99.1.2 5.99.1.2] </span></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6cq2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6cq2 OCA], [http://pdbe.org/6cq2 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6cq2 RCSB], [http://www.ebi.ac.uk/pdbsum/6cq2 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6cq2 ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[[http://www.uniprot.org/uniprot/TOP1_MYCTU TOP1_MYCTU]] Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.[HAMAP-Rule:MF_00952]<ref>PMID:20724443</ref> The C-terminus (residues 622-934) inhibits RNA cleavage by MazF4.<ref>PMID:20724443</ref> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: DNA topoisomerase]] | |||
[[Category: Cao, N]] | |||
[[Category: Tan, K]] | [[Category: Tan, K]] | ||
[[Category: Thirunavukkaraus, A]] | [[Category: Thirunavukkaraus, A]] | ||
[[Category: Tse-Dinh, Y | [[Category: Tse-Dinh, Y C]] | ||
[[Category: | [[Category: Co-crystallization]] | ||
[[Category: Complex with oligonucleotide and magnesium]] | |||
[[Category: Isomerase]] | |||
[[Category: Isomerase-dna complex]] | |||
[[Category: Mycobacterium tuberculosis]] | |||
[[Category: Topoisomerase i]] | |||
Revision as of 05:35, 30 May 2018
Crystal structure of Mycobacterium tuberculosis Topoisomerase I in complex with oligonucleotide MTS2-12 and Magnesium
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