6c40: Difference between revisions
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==CheY41PyTyrD54K from Thermotoga maritima== | |||
<StructureSection load='6c40' size='340' side='right' caption='[[6c40]], [[Resolution|resolution]] 2.70Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[6c40]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6C40 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6C40 FirstGlance]. <br> | |||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene></td></tr> | |||
<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=0WZ:3-(1H-PYRAZOL-1-YL)-L-TYROSINE'>0WZ</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6c40 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6c40 OCA], [http://pdbe.org/6c40 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6c40 RCSB], [http://www.ebi.ac.uk/pdbsum/6c40 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6c40 ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[[http://www.uniprot.org/uniprot/CHEY_THEMA CHEY_THEMA]] Involved in the transmission of sensory signals from the chemoreceptors to the flagellar motors. CheY seems to regulate the clockwise (CW) rotation (By similarity). | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Pulsed dipolar electron spin resonance spectroscopy (PDS) is a powerful tool for measuring distances in solution-state macromolecules. Paramagnetic metal ions, such as Cu(2+), are used as spin probes because they can report on metalloprotein features and can be spectroscopically distinguished from traditional nitroxide (NO)-based labels. Here, we demonstrate site-specific incorporation of Cu(2+) into non-metalloproteins through the use of a genetically encodable non-natural amino acid, 3-pyrazolyltyrosine (PyTyr). We first incorporate PyTyr in cyan fluorescent protein to measure Cu(2+)-to-NO distances and examine the effects of solvent conditions on Cu(2+) binding and protein aggregation. We then apply the method to characterize the complex formed by the histidine kinase CheA and its target response regulator CheY. The X-ray structure of CheY-PyTyr confirms Cu labeling at PyTyr but also reveals a secondary Cu site. Cu(2+)-to-NO and Cu(2+)-to-Cu(2+) PDS measurements of CheY-PyTyr with nitroxide-labeled CheA provide new insights into the conformational landscape of the phosphotransfer complex and have implications for kinase regulation. | |||
Site-Specific Incorporation of a Cu(2+) Spin Label into Proteins for Measuring Distances by Pulsed Dipolar Electron Spin Resonance Spectroscopy.,Merz GE, Borbat PP, Muok AR, Srivastava M, Bunck DN, Freed JH, Crane BR J Phys Chem B. 2018 Oct 3. doi: 10.1021/acs.jpcb.8b05619. PMID:30222354<ref>PMID:30222354</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: Muok, A | <div class="pdbe-citations 6c40" style="background-color:#fffaf0;"></div> | ||
[[Category: | == References == | ||
[[Category: | <references/> | ||
__TOC__ | |||
</StructureSection> | |||
[[Category: Crane, B R]] | |||
[[Category: Merz, G E]] | |||
[[Category: Muok, A R]] | |||
[[Category: Chemotaxis]] | |||
[[Category: Chey]] | |||
[[Category: Pytyr]] | |||
[[Category: Signaling protein]] | |||
