6cw7: Difference between revisions

From Proteopedia
Jump to navigationJump to search
← Older editNewer edit →
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,328,572 edits
No edit summary
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,328,572 edits
No edit summary
Line 1: Line 1:
'''Unreleased structure'''


The entry 6cw7 is ON HOLD  until Paper Publication
==E. coli DHFR product complex with (6S)-5,6,7,8-TETRAHYDROFOLATE==
<StructureSection load='6cw7' size='340' side='right' caption='[[6cw7]], [[Resolution|resolution]] 1.03&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6cw7]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6CW7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6CW7 FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=THG:(6S)-5,6,7,8-TETRAHYDROFOLATE'>THG</scene></td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[6cxk|6cxk]], [[6cqa|6cqa]]</td></tr>
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Dihydrofolate_reductase Dihydrofolate reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.5.1.3 1.5.1.3] </span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6cw7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6cw7 OCA], [http://pdbe.org/6cw7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6cw7 RCSB], [http://www.ebi.ac.uk/pdbsum/6cw7 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6cw7 ProSAT]</span></td></tr>
</table>
== Function ==
[[http://www.uniprot.org/uniprot/DYR_ECOL6 DYR_ECOL6]] Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis (By similarity).
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Dihydrofolate reductase (DHFR) catalyzes the stereospecific reduction of 7,8-dihydrofolate (FH2) to (6s)-5,6,7,8-tetrahydrofolate (FH4) via hydride transfer from NADPH. The consensus Escherichia coli DHFR mechanism involves conformational changes between closed and occluded states occurring during the rate-limiting product release step. Although the Protein Data Bank (PDB) contains over 250 DHFR structures, the FH4 complex structure responsible for rate-limiting product release is unknown. We report to our knowledge the first crystal structure of an E. coli. DHFR:FH4 complex at 1.03 A resolution showing distinct stabilizing interactions absent in FH2 or related (6R)-5,10-dideaza-FH4 complexes. We discover the time course of decay of the co-purified endogenous FH4 during crystal growth, with conversion from FH4 to FH2 occurring in 2-3 days. We also determine another occluded complex structure of E. coli DHFR with a slow-onset nanomolar inhibitor that contrasts with the methotrexate complex, suggesting a plausible strategy for designing DHFR antibiotics by targeting FH4 product conformations.


Authors: Cao, H., Rodrigues, J., Benach, J., Frommelt, A., Morisco, L., Koss, J., Shakhnovich, E., Skolnick, J.
The crystal structure of a tetrahydrofolate-bound dihydrofolate reductase reveals the origin of slow product release.,Cao H, Gao M, Zhou H, Skolnick J Commun Biol. 2018 Dec 12;1:226. doi: 10.1038/s42003-018-0236-y. eCollection 2018. PMID:30564747<ref>PMID:30564747</ref>


Description: E. coli DHFR product complex with (6S)-5,6,7,8-TETRAHYDROFOLATE
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
[[Category: Unreleased Structures]]
</div>
<div class="pdbe-citations 6cw7" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Dihydrofolate reductase]]
[[Category: Benach, J]]
[[Category: Cao, H]]
[[Category: Frommelt, A]]
[[Category: Koss, J]]
[[Category: Koss, J]]
[[Category: Morisco, L]]
[[Category: Morisco, L]]
[[Category: Frommelt, A]]
[[Category: Rodrigues, J]]
[[Category: Shakhnovich, E]]
[[Category: Shakhnovich, E]]
[[Category: Rodrigues, J]]
[[Category: Benach, J]]
[[Category: Skolnick, J]]
[[Category: Skolnick, J]]
[[Category: Cao, H]]
[[Category: Complex]]
[[Category: Dhfr]]
[[Category: Oxidoreductase]]
[[Category: Tetrahydrofolate]]
Retrieved from "https://proteopedia.org/6cw7"