2h3e: Difference between revisions

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[[Image:2h3e.gif|left|200px]]
[[Image:2h3e.gif|left|200px]]


{{Structure
<!--
|PDB= 2h3e |SIZE=350|CAPTION= <scene name='initialview01'>2h3e</scene>, resolution 2.300&Aring;
The line below this paragraph, containing "STRUCTURE_2h3e", creates the "Structure Box" on the page.
|SITE=
You may change the PDB parameter (which sets the PDB file loaded into the applet)  
|LIGAND= <scene name='pdbligand=6PR:(S)-4-AMINO-4-OXO-3-(2-PHOSPHONOACETAMIDO)BUTANOIC+ACID'>6PR</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Aspartate_carbamoyltransferase Aspartate carbamoyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.3.2 2.1.3.2] </span>
or leave the SCENE parameter empty for the default display.
|GENE= pyrB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli]), pyrI ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])
-->
|DOMAIN=
{{STRUCTURE_2h3e| PDB=2h3e  | SCENE= }}  
|RELATEDENTRY=[[1d09|1D09]]
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2h3e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2h3e OCA], [http://www.ebi.ac.uk/pdbsum/2h3e PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2h3e RCSB]</span>
}}


'''Structure of wild-type E. coli Aspartate Transcarbamoylase in the presence of N-phosphonacetyl-L-isoasparagine at 2.3A resolution'''
'''Structure of wild-type E. coli Aspartate Transcarbamoylase in the presence of N-phosphonacetyl-L-isoasparagine at 2.3A resolution'''
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[[Category: Tsuruta, H.]]
[[Category: Tsuruta, H.]]
[[Category: Xia, J.]]
[[Category: Xia, J.]]
[[Category: cooperativity]]
[[Category: Cooperativity]]
[[Category: x-ray crystallography]]
[[Category: X-ray crystallography]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 05:49:15 2008''
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:24:54 2008''

Revision as of 02:49, 4 May 2008

File:2h3e.gif

Template:STRUCTURE 2h3e

Structure of wild-type E. coli Aspartate Transcarbamoylase in the presence of N-phosphonacetyl-L-isoasparagine at 2.3A resolution


Overview

The synthesis of a new inhibitor, N-phosphonacetyl-L-isoasparagine (PALI), of Escherichia coli aspartate transcarbamoylase (ATCase) is reported, as well as structural studies of the enzyme.PALI complex. PALI was synthesized in 7 steps from beta-benzyl L-aspartate. The KD of PALI was 2 microM. Kinetics and small-angle X-ray scattering experiments showed that PALI can induce the cooperative transition of ATCase from the T to the R state. The X-ray structure of the enzyme.PALI complex showed 22 hydrogen-bonding interactions between the enzyme and PALI. The kinetic characterization and crystal structure of the ATCase.PALI complex also provides detailed information regarding the importance of the alpha-carboxylate for the binding of the substrate aspartate.

About this Structure

2H3E is a Protein complex structure of sequences from Escherichia coli. Full crystallographic information is available from OCA.

Reference

N-phosphonacetyl-L-isoasparagine a potent and specific inhibitor of Escherichia coli aspartate transcarbamoylase., Eldo J, Cardia JP, O'Day EM, Xia J, Tsuruta H, Kantrowitz ER, J Med Chem. 2006 Oct 5;49(20):5932-8. PMID:17004708 Page seeded by OCA on Sun May 4 05:49:15 2008

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