2hl9: Difference between revisions
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'''SUMO protease Ulp1 with the catalytic cysteine oxidized to a sulfonic acid''' | '''SUMO protease Ulp1 with the catalytic cysteine oxidized to a sulfonic acid''' | ||
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[[Category: Ng, T B.]] | [[Category: Ng, T B.]] | ||
[[Category: Xu, Z.]] | [[Category: Xu, Z.]] | ||
[[Category: | [[Category: Hydrolase]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 06:25:25 2008'' | |||
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Revision as of 03:25, 4 May 2008
SUMO protease Ulp1 with the catalytic cysteine oxidized to a sulfonic acid
Overview
Modification of cellular proteins by the ubiquitin-like protein SUMO is essential for nuclear processes and cell cycle progression in yeast. The Ulp1 protease catalyzes two essential functions in the SUMO pathway: (1) processing of full-length SUMO to its mature form and (2) deconjugation of SUMO from targeted proteins. Selective reduction of the proteolytic reaction produced a covalent thiohemiacetal transition state complex between a Ulp1 C-terminal fragment and its cellular substrate Smt3, the yeast SUMO homolog. The Ulp1-Smt3 crystal structure and functional testing of elements within the conserved interface elucidate determinants of SUMO recognition, processing, and deconjugation. Genetic analysis guided by the structure further reveals a regulatory element N-terminal to the proteolytic domain that is required for cell growth in yeast.
About this Structure
2HL9 is a Single protein structure of sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.
Reference
Ulp1-SUMO crystal structure and genetic analysis reveal conserved interactions and a regulatory element essential for cell growth in yeast., Mossessova E, Lima CD, Mol Cell. 2000 May;5(5):865-76. PMID:10882122 Page seeded by OCA on Sun May 4 06:25:25 2008
