6cl3: Difference between revisions
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The | ==LyeTxI-b, a synthetic peptide derived from Lycosa erythrognatha spider venom, shows potent antibiotic activity, in vitro and in vivo== | ||
<StructureSection load='6cl3' size='340' side='right' caption='[[6cl3]], [[NMR_Ensembles_of_Models | 10 NMR models]]' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[6cl3]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6CL3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6CL3 FirstGlance]. <br> | |||
</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene>, <scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6cl3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6cl3 OCA], [http://pdbe.org/6cl3 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6cl3 RCSB], [http://www.ebi.ac.uk/pdbsum/6cl3 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6cl3 ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The antimicrobial peptide LyeTxI isolated from the venom of the spider Lycosa erythrognatha is a potential model to develop new antibiotics against bacteria and fungi. In this work, we studied a peptide derived from LyeTxI, named LyeTxI-b, and characterized its structural profile and its in vitro and in vivo antimicrobial activities. Compared to LyeTxI, LyeTxI-b has an acetylated N-terminal and a deletion of a His residue, as structural modifications. The secondary structure of LyeTxI-b is a well-defined helical segment, from the second amino acid to the amidated C-terminal, with no clear partition between hydrophobic and hydrophilic faces. Moreover, LyeTxI-b shows a potent antimicrobial activity against Gram-positive and Gram-negative planktonic bacteria, being 10-fold more active than the native peptide against Escherichia coli. LyeTxI-b was also active in an in vivo model of septic arthritis, reducing the number of bacteria load, the migration of immune cells, the level of IL-1beta cytokine and CXCL1 chemokine, as well as preventing cartilage damage. Our results show that LyeTxI-b is a potential therapeutic model for the development of new antibiotics against Gram-positive and Gram-negative bacteria. | |||
LyeTxI-b, a Synthetic Peptide Derived From Lycosa erythrognatha Spider Venom, Shows Potent Antibiotic Activity in Vitro and in Vivo.,Reis PVM, Boff D, Verly RM, Melo-Braga MN, Cortes ME, Santos DM, Pimenta AMC, Amaral FA, Resende JM, de Lima ME Front Microbiol. 2018 Apr 6;9:667. doi: 10.3389/fmicb.2018.00667. eCollection, 2018. PMID:29681894<ref>PMID:29681894</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: | <div class="pdbe-citations 6cl3" style="background-color:#fffaf0;"></div> | ||
[[Category: Resende, J | == References == | ||
[[Category: Verly, R | <references/> | ||
[[Category: | __TOC__ | ||
</StructureSection> | |||
[[Category: Lima, M E.de]] | |||
[[Category: Reis, P V.dos]] | |||
[[Category: Resende, J M]] | |||
[[Category: Verly, R M]] | |||
[[Category: Antimicrobial peptide]] | |||
[[Category: Antimicrobial protein]] | |||
