6cvm: Difference between revisions
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==Atomic resolution cryo-EM structure of beta-galactosidase== | |||
<StructureSection load='6cvm' size='340' side='right' caption='[[6cvm]], [[Resolution|resolution]] 1.90Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[6cvm]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli_(strain_k12) Escherichia coli (strain k12)]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6CVM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6CVM FirstGlance]. <br> | |||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=PTQ:2-PHENYLETHYL+1-THIO-BETA-D-GALACTOPYRANOSIDE'>PTQ</scene></td></tr> | |||
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Beta-galactosidase Beta-galactosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.23 3.2.1.23] </span></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6cvm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6cvm OCA], [http://pdbe.org/6cvm PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6cvm RCSB], [http://www.ebi.ac.uk/pdbsum/6cvm PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6cvm ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The advent of direct electron detectors has enabled the routine use of single-particle cryo-electron microscopy (EM) approaches to determine structures of a variety of protein complexes at near-atomic resolution. Here, we report the development of methods to account for local variations in defocus and beam-induced drift, and the implementation of a data-driven dose compensation scheme that significantly improves the extraction of high-resolution information recorded during exposure of the specimen to the electron beam. These advances enable determination of a cryo-EM density map for beta-galactosidase bound to the inhibitor phenylethyl beta-D-thiogalactopyranoside where the ordered regions are resolved at a level of detail seen in X-ray maps at approximately 1.5 A resolution. Using this density map in conjunction with constrained molecular dynamics simulations provides a measure of the local flexibility of the non-covalently bound inhibitor and offers further opportunities for structure-guided inhibitor design. | |||
Atomic Resolution Cryo-EM Structure of beta-Galactosidase.,Bartesaghi A, Aguerrebere C, Falconieri V, Banerjee S, Earl LA, Zhu X, Grigorieff N, Milne JLS, Sapiro G, Wu X, Subramaniam S Structure. 2018 May 10. pii: S0969-2126(18)30129-1. doi:, 10.1016/j.str.2018.04.004. PMID:29754826<ref>PMID:29754826</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
<div class="pdbe-citations 6cvm" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Beta-galactosidase]] | |||
[[Category: Banerjee, S]] | |||
[[Category: Bartesaghi, A]] | |||
[[Category: Milne, J L.S]] | |||
[[Category: Subramaniam, S]] | [[Category: Subramaniam, S]] | ||
[[Category: Zhu, X]] | [[Category: Zhu, X]] | ||
[[Category: | [[Category: Computer-aided drug discovery]] | ||
[[Category: | [[Category: Drift correction]] | ||
[[Category: | [[Category: Drug discovery]] | ||
[[Category: Hydrolase]] | |||
[[Category: Precision medicine]] | |||
[[Category: Radiation damage]] | |||
Revision as of 05:36, 30 May 2018
Atomic resolution cryo-EM structure of beta-galactosidase
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