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'''Crystal structure of the B30.2/SPRY domain of GUSTAVUS in complex with a 20-residue VASA peptide''' | '''Crystal structure of the B30.2/SPRY domain of GUSTAVUS in complex with a 20-residue VASA peptide''' | ||
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[[Category: Park, S Y.]] | [[Category: Park, S Y.]] | ||
[[Category: Woo, J S.]] | [[Category: Woo, J S.]] | ||
[[Category: | [[Category: B30 2/spry]] | ||
[[Category: | [[Category: F-box-spry]] | ||
[[Category: | [[Category: Gustavus]] | ||
[[Category: | [[Category: Spry-containing socs box]] | ||
[[Category: | [[Category: Trim family]] | ||
[[Category: | [[Category: Vasa]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 07:31:21 2008'' | |||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | |||
Revision as of 04:31, 4 May 2008
Crystal structure of the B30.2/SPRY domain of GUSTAVUS in complex with a 20-residue VASA peptide
Overview
B30.2/SPRY domains are found in numerous proteins that cover a wide spectrum of biological functions, including regulation of cytokine signaling and innate retroviral restriction. Herein, we report the crystal structure of the B30.2/SPRY domain of a SPRY domain-containing SOCS box (SSB) protein, GUSTAVUS, complexed with a 20 amino acid peptide derived from the RNA helicase VASA, revealing how these domains recognize target proteins. The peptide-binding site is conformationally rigid and has a preformed pocket. The interaction between the pocket and the Asp-Ile-Asn-Asn-Asn-Asn sequence within the peptide accounts for the high-affinity binding between GUSTAVUS and VASA. This observation led to a facile identification of the Glu-Leu-Asn-Asn-Asn-Leu sequence as the recognition motif in a proapoptotic protein Par-4 for its interaction with a GUSTAVUS homolog, SSB-1. Ensuing analyses indicated that many B30.2/SPRY domains have a similar preformed pocket, which would allow them to bind multiple targets.
About this Structure
2IHS is a Protein complex structure of sequences from Drosophila melanogaster. Full crystallographic information is available from OCA.
Reference
Structural basis for protein recognition by B30.2/SPRY domains., Woo JS, Suh HY, Park SY, Oh BH, Mol Cell. 2006 Dec 28;24(6):967-76. PMID:17189197 Page seeded by OCA on Sun May 4 07:31:21 2008
