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| ==Crystal structure of protein DR0370 from Deinococcus radiodurans, Pfam DUF178== | | ==Crystal structure of protein DR0370 from Deinococcus radiodurans, Pfam DUF178== |
| <StructureSection load='2i6e' size='340' side='right' caption='[[2i6e]], [[Resolution|resolution]] 2.50Å' scene=''> | | <StructureSection load='2i6e' size='340' side='right'caption='[[2i6e]]' scene=''> |
| == Structural highlights == | | == Structural highlights == |
| <table><tr><td colspan='2'>[[2i6e]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/"micrococcus_radiodurans"_raj_et_al._1960 "micrococcus radiodurans" raj et al. 1960]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2I6E OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2I6E FirstGlance]. <br> | | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2I6E OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2I6E FirstGlance]. <br> |
| </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2i6e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2i6e OCA], [https://pdbe.org/2i6e PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2i6e RCSB], [https://www.ebi.ac.uk/pdbsum/2i6e PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2i6e ProSAT], [https://www.topsan.org/Proteins/NYSGXRC/2i6e TOPSAN]</span></td></tr> |
| <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
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| <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">DR_0370 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1299 "Micrococcus radiodurans" Raj et al. 1960])</td></tr>
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| <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2i6e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2i6e OCA], [http://pdbe.org/2i6e PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2i6e RCSB], [http://www.ebi.ac.uk/pdbsum/2i6e PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2i6e ProSAT], [http://www.topsan.org/Proteins/NYSGXRC/2i6e TOPSAN]</span></td></tr> | |
| </table> | | </table> |
| == Function ==
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| [[http://www.uniprot.org/uniprot/Q9RXE3_DEIRA Q9RXE3_DEIRA]] Catalyzes the dehydration of chorismate into 3-[(1-carboxyvinyl)oxy]benzoate, a step in the biosynthesis of menaquinone (MK, vitamin K2).[HAMAP-Rule:MF_00995]
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| == Evolutionary Conservation == | | == Evolutionary Conservation == |
| [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2i6e ConSurf]. | | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2i6e ConSurf]. |
| <div style="clear:both"></div> | | <div style="clear:both"></div> |
| <div style="background-color:#fffaf0;">
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| == Publication Abstract from PubMed ==
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| BACKGROUND: Pfam is a comprehensive collection of protein domains and families, with a range of well-established information including genome annotation. Pfam has two large series of functionally uncharacterized families, known as Domains of Unknown Function (DUFs) and Uncharacterized Protein Families (UPFs). RESULTS: Crystal structures of two proteins from Deinococcus radiodurans and Streptomyces coelicolor belonging to Pfam protein family DUF178 (ID: PF02621) have been determined using Selenium-Single-wavelength Anomalous Dispersion (Se-SAD). Based on the structure, we have identified the putative function for this family of protein. CONCLUSION: Unexpectedly, we found that DUF178 Pfam is remarkably similar to Pfam family DUF191 suggesting that the sequence-based classification alone may not be sufficient to classify proteins into Pfam families.
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| X-ray structures of two proteins belonging to Pfam DUF178 revealed unexpected structural similarity to the DUF191 Pfam family.,Tyagi R, Burley SK, Swaminathan S BMC Struct Biol. 2007 Oct 1;7:62. PMID:17908300<ref>PMID:17908300</ref>
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| From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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| </div>
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| <div class="pdbe-citations 2i6e" style="background-color:#fffaf0;"></div>
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| == References ==
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| <references/>
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| __TOC__ | | __TOC__ |
| </StructureSection> | | </StructureSection> |
| [[Category: Micrococcus radiodurans raj et al. 1960]] | | [[Category: Large Structures]] |
| [[Category: Burley, S K]] | | [[Category: Burley SK]] |
| [[Category: Kumaran, D]] | | [[Category: Kumaran D]] |
| [[Category: Structural genomic]]
| | [[Category: Swaminathan S]] |
| [[Category: Swaminathan, S]] | | [[Category: Tyagi R]] |
| [[Category: Tyagi, R]] | |
| [[Category: Hypothetical protein]]
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| [[Category: NYSGXRC, New York SGX Research Center for Structural Genomics]]
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| [[Category: Pfam:duf178]]
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| [[Category: PSI, Protein structure initiative]]
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| [[Category: Unknown function]]
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