2kn4

The structure of the RRM domain of SC35

spinqualitylabelsall models 2kn4, 20 NMR models Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 2kn4 is a 1 chain structure with sequence from Strsg. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance. Gene: SRSF2, SFRS2 (STRSG) Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMed SRSF2 is a prototypical SR protein which plays important roles in the alternative splicing of pre-mRNA. It has been shown to be involved in regulatory pathways for maintaining genomic stability and play important roles in regulating key receptors in the heart. We report here the solution structure of the RNA recognition motifs (RRM) domain of free human SRSF2 (residues 9-101). Compared with other members of the SR protein family, SRSF2 structure has a longer L3 loop region. The conserved aromatic residue in the RNP2 motif is absent in SRSF2. Calorimetric titration shows that the RNA sequence 5'AGCAGAGUA3' binds SRSF2 with a K(d) of 61 +/- 1 nM and a 1:1 stoichiometry. NMR and mutagenesis experiments reveal that for SFSF2, the canonical beta1 and beta3 interactions are themselves not sufficient for effective RNA binding; the additional loop L3 is crucial for RNA complex formation. A comparison is made between the structures of SRSF2-RNA complex with other known RNA complexes of SR proteins. We conclude that interactions involving the L3 loop, N- and C-termini of the RRM domain are collectively important for determining selectivity between the protein and RNA. The structure and selectivity of the SR protein SRSF2 RRM domain with RNA.,Phelan MM, Goult BT, Clayton JC, Hautbergue GM, Wilson SA, Lian LY Nucleic Acids Res. 2011 Dec 2. PMID:22140111 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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