Introduction to Evolutionary Conservation: Difference between revisions
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<center>{{Template:ColorKey_ConSurf_NoGray}}</center> | <center>{{Template:ColorKey_ConSurf_NoGray}}</center> | ||
====Sequence Colored by Conservation==== | |||
When ConSurf's colors are applied to the 436 amino acids in the sequence of enolase (based on a multiple sequence alignment containing 150 sequences), this is the result: | When ConSurf's colors are applied to the 436 amino acids in the sequence of enolase (based on a multiple sequence alignment containing 150 sequences), this is the result: | ||
[[Image:4enl consurf150 sequence wb.jpg|400 px|left]] | [[Image:4enl consurf150 sequence wb.jpg|400 px|left]] | ||
{{Clear}} | {{Clear}} | ||
Notice that the conserved residues are scattered around the sequence with no obvious pattern. However, when the same <scene name='Introduction_to_Evolutionary_Conservation/Enolase_with_consurf_colors/1'>colors are applied to the amino acids in the 3D structure</scene>, they form a conserved patch around the catalytic site (marked with a <span style="background:black; color:#00ff00;">''' zinc ion colored green '''</span>. | Notice that the conserved residues are scattered around the sequence with no obvious pattern. | ||
====3D Structure Colored by Conservation==== | |||
However, when the same <scene name='Introduction_to_Evolutionary_Conservation/Enolase_with_consurf_colors/1'>colors are applied to the amino acids in the 3D structure</scene>, they form a conserved patch around the catalytic site (marked with a <span style="background:black; color:#00ff00;">''' zinc ion colored green '''</span>. | |||
*Show [http://firstglance.jmol.org/fg.htm?mol=http%3A//bioinformatics.org/firstglance/fgij/localPDBFiles/4ENLA_ConSurf_DB_pipe.pdb.gz conservation of enolase in FirstGlance in Jmol] ([[4enl]]). | *Show [http://firstglance.jmol.org/fg.htm?mol=http%3A//bioinformatics.org/firstglance/fgij/localPDBFiles/4ENLA_ConSurf_DB_pipe.pdb.gz conservation of enolase in FirstGlance in Jmol] ([[4enl]]). | ||
