2maw

NMR structures of the alpha7 nAChR transmembrane domain.

spinqualitylabelsall models 2maw, 20 NMR models Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 2maw is a 1 chain structure with sequence from Human. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance. Gene: CHRFAM7A, CHRNA7, NACHRA7 (HUMAN) Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Disease [ACHA7_HUMAN] 15q13.3 microdeletion syndrome. Function [ACHA7_HUMAN] After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. The channel is blocked by alpha-bungarotoxin. Publication Abstract from PubMed Nicotinic acetylcholine receptors (nAChRs) are targets of general anesthetics, but functional sensitivity to anesthetic inhibition varies dramatically among different subtypes of nAChRs. Potential causes underlying different functional responses to anesthetics remain elusive. Here we show that in contrast to the alpha7 nAChR, the alpha7beta2 nAChR is highly susceptible to inhibition by the volatile anesthetic isoflurane in electrophysiology measurements. Isoflurane-binding sites in beta2 and alpha7 were found at the extracellular and intracellular end of their respective transmembrane domains using NMR. Functional relevance of the identified beta2 site was validated via point mutations and subsequent functional measurements. Consistent with their functional responses to isoflurane, beta2 but not alpha7 showed pronounced dynamics changes, particularly for the channel gate residue Leu-249(9'). These results suggest that anesthetic binding alone is not sufficient to generate functional impact; only those sites that can modulate channel dynamics upon anesthetic binding will produce functional effects. Insights into distinct modulation of alpha7 and alpha7beta2 nicotinic acetylcholine receptors by the volatile anesthetic isoflurane.,Mowrey DD, Liu Q, Bondarenko V, Chen Q, Seyoum E, Xu Y, Wu J, Tang P J Biol Chem. 2013 Dec 13;288(50):35793-800. doi: 10.1074/jbc.M113.508333. Epub, 2013 Nov 5. PMID:24194515 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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