2mgv

NMR structure of PASTA domain of PonA2 from Mycobacterium tuberculosis

spinqualitylabelsall models 2mgv, 10 NMR models Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 2mgv is a 1 chain structure with sequence from Myctu. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance. Gene: RVBD_3682 (MYCTU) Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed PonA2 is one of the two class A Penicillin binding proteins of Mycobacterium tuberculosis, the etiologic agent of tuberculosis. It plays a complex role in mycobacterial physiology and is spotted as a promising target for inhibitors. PonA2 is involved in adaptation of M. tuberculosis to dormancy, an ability which has been attributed to the presence in its sequence of a C-terminal PASTA domain. Since PASTA modules are typically considered as beta-lactam antibiotic binding domains, we determined the solution structure of the PASTA domain from PonA2 and analysed its binding properties versus a plethora of potential binders, including the beta-lactam antibiotics, two typical muropeptide mimics and polymeric peptidoglycan. We show that, despite a high structural similarity with other PASTA domains, the PASTA domain of PonA2 displays different binding properties, as it is not able to bind muropeptides, nor beta-lactams, nor polymeric peptidoglycan. These results indicate that the role of PASTA domains cannot be generalized, as their specific binding properties strongly depend on surface residues, which are widely variable. Structural and binding properties of the PASTA domain of PonA2, a key penicillin binding protein from Mycobacterium tuberculosis.,Calvanese L, Falcigno L, Maglione C, Marasco D, Ruggiero A, Squeglia F, Berisio R, D'Auria G Biopolymers. 2013 Nov 27. doi: 10.1002/bip.22447. PMID:24281824 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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