6aa9: Difference between revisions
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==T166A mutant of D-Serine deaminase from Salmonella typhimurium== | |||
<StructureSection load='6aa9' size='340' side='right'caption='[[6aa9]], [[Resolution|resolution]] 2.70Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[6aa9]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6AA9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6AA9 FirstGlance]. <br> | |||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr> | |||
<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=LLP:(2S)-2-AMINO-6-[[3-HYDROXY-2-METHYL-5-(PHOSPHONOOXYMETHYL)PYRIDIN-4-YL]METHYLIDENEAMINO]HEXANOIC+ACID'>LLP</scene></td></tr> | |||
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/D-serine_ammonia-lyase D-serine ammonia-lyase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.3.1.18 4.3.1.18] </span></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6aa9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6aa9 OCA], [http://pdbe.org/6aa9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6aa9 RCSB], [http://www.ebi.ac.uk/pdbsum/6aa9 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6aa9 ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Enteric pathogens such as Salmonella typhimurium colonize the human gut in spite of the lethal acidic pH environment (pH < 2.5) due to the activation of inducible acid tolerance response (ATR) systems. The pyridoxal 5'-phosphate (PLP)-dependent enzyme, biodegradative arginine decarboxylase (ADC, encoded by AdiA), is a component of an ATR system. The enzyme consumes a cytoplasmic proton in the process of arginine degradation to agmatine. Arginine-agmatine antiporter (AdiC) exchanges the product agmatine for arginine. In this manuscript, we describe the structure of Salmonella typhimurium ADC (StADC). The decameric structure assembled from five dimers related by a non crystallographic 5-fold symmetry represents the first apo-form of the enzyme. The structure suggests that PLP-binding is not a prerequisite for oligomerization. Comparison with E. coli ADC reveals that PLP-binding is accompanied by the movement and ordering of two loops (residues 150-159 and 191-197) and a few active site residues such as His256 and Lys257. A number of residues important for substrate binding are disordered in the apo-StADC structure indicating that PLP binding is important for substrate binding. Unlike the interactions between 5-fold related protomers, interactions that stabilize the dimeric structure are not pH dependent. | |||
Structural studies on the decameric S. typhimurium arginine decarboxylase (ADC): Pyridoxal 5'-phosphate binding induces conformational changes.,Deka G, Bharath SR, Savithri HS, Murthy MRN Biochem Biophys Res Commun. 2017 Sep 2;490(4):1362-1368. doi:, 10.1016/j.bbrc.2017.07.032. Epub 2017 Jul 8. PMID:28694189<ref>PMID:28694189</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: Bharath, S | <div class="pdbe-citations 6aa9" style="background-color:#fffaf0;"></div> | ||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: D-serine ammonia-lyase]] | |||
[[Category: Large Structures]] | |||
[[Category: Bharath, S R]] | |||
[[Category: Deka, G]] | [[Category: Deka, G]] | ||
[[Category: Murthy, M | [[Category: Murthy, M R.N]] | ||
[[Category: Shavithri, H | [[Category: Shavithri, H S]] | ||
[[Category: Deaminase]] | |||
[[Category: Lyase]] | |||
[[Category: Monomer]] | |||
[[Category: Plp bound]] | |||
[[Category: Salmonella typhimurium]] | |||
Revision as of 10:35, 17 July 2019
T166A mutant of D-Serine deaminase from Salmonella typhimurium
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