2pns: Difference between revisions
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
[[Image:2pns.jpg|left|200px]] | [[Image:2pns.jpg|left|200px]] | ||
<!-- | |||
The line below this paragraph, containing "STRUCTURE_2pns", creates the "Structure Box" on the page. | |||
You may change the PDB parameter (which sets the PDB file loaded into the applet) | |||
or the SCENE parameter (which sets the initial scene displayed when the page is loaded), | |||
or leave the SCENE parameter empty for the default display. | |||
--> | |||
| | {{STRUCTURE_2pns| PDB=2pns | SCENE= }} | ||
| | |||
}} | |||
'''1.9 Angstrom resolution crystal structure of a plant cysteine protease Ervatamin-C refinement with cDNA derived amino acid sequence''' | '''1.9 Angstrom resolution crystal structure of a plant cysteine protease Ervatamin-C refinement with cDNA derived amino acid sequence''' | ||
| Line 19: | Line 16: | ||
==About this Structure== | ==About this Structure== | ||
Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PNS OCA]. | |||
==Reference== | ==Reference== | ||
A thermostable cysteine protease precursor from a tropical plant contains an unusual C-terminal propeptide: cDNA cloning, sequence comparison and molecular modeling studies., Ghosh R, Dattagupta JK, Biswas S, Biochem Biophys Res Commun. 2007 Nov 3;362(4):965-70. Epub 2007 Aug 28. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17767923 17767923] | A thermostable cysteine protease precursor from a tropical plant contains an unusual C-terminal propeptide: cDNA cloning, sequence comparison and molecular modeling studies., Ghosh R, Dattagupta JK, Biswas S, Biochem Biophys Res Commun. 2007 Nov 3;362(4):965-70. Epub 2007 Aug 28. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17767923 17767923] | ||
[[Category: Biswas, S.]] | [[Category: Biswas, S.]] | ||
[[Category: Chakrabarti, C.]] | [[Category: Chakrabarti, C.]] | ||
| Line 30: | Line 25: | ||
[[Category: Ghosh, R.]] | [[Category: Ghosh, R.]] | ||
[[Category: Thakurta, P Guha.]] | [[Category: Thakurta, P Guha.]] | ||
[[Category: | [[Category: Ervatamin]] | ||
[[Category: | [[Category: Hydrolase]] | ||
[[Category: | [[Category: Papain-like fold]] | ||
[[Category: | [[Category: Plant cysteine protease]] | ||
[[Category: | [[Category: Thermostable]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 13:29:55 2008'' | |||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | |||
Revision as of 10:29, 4 May 2008
1.9 Angstrom resolution crystal structure of a plant cysteine protease Ervatamin-C refinement with cDNA derived amino acid sequence
Overview
We report here the cloning and characterization of the entire cDNA of a papain-like cysteine protease from a tropical flowering plant. The 1098-bp ORF of the cDNA codify a protease precursor having a signal peptide of 19 amino acids, a cathepsin-L like N-terminal proregion of 114 amino acids, a mature enzyme part of 208 amino acids and a C-terminal proregion of 24 amino acids. The derived amino acid sequence of the mature part tallies with the thermostable cysteine protease Ervatamin-C--as was aimed at. The C-terminal proregion of the protease has altogether a different sequence pattern not observed in other members of the family and it contains a negatively charged helical zone. The three-dimensional model of the precursor, based on the homology modeling and X-ray structure, shows that the extended peptide stretch region of the N-terminal propeptide, covering the interdomain cleft, contains protruding side chains of positively charged residues. This study also indicates that the negatively charged zone of C-terminal propeptide may interact with the positively charged zone of the N-terminal propeptide in a cooperative manner in the maturation process of this enzyme.
About this Structure
Full crystallographic information is available from OCA.
Reference
A thermostable cysteine protease precursor from a tropical plant contains an unusual C-terminal propeptide: cDNA cloning, sequence comparison and molecular modeling studies., Ghosh R, Dattagupta JK, Biswas S, Biochem Biophys Res Commun. 2007 Nov 3;362(4):965-70. Epub 2007 Aug 28. PMID:17767923 Page seeded by OCA on Sun May 4 13:29:55 2008
