6e0a

Crystal Structure of Helicobacter pylori TlpA Chemoreceptor Ligand Binding Domain

spinqualitylabelsall models 6e0a, resolution 2.43Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 6e0a is a 2 chain structure with sequence from Helicobacter pylori (strain ss1). Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: CA, CL, DTT, ZN Gene: tlpA, HPYLSS1_00094 (Helicobacter pylori (strain SS1)) Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed Bacteria use chemoreceptor proteins to sense and navigate their chemical environments. The most common class of chemoreceptors are transmembrane proteins that sense chemical cues through binding of a small molecule ligand to a periplasmic domain, which modulates the receptor's ability to stimulate reversal of the cell's flagella motors. The prevalent gastric pathogen Helicobacter pylori (H. pylori) uses such membrane-bound chemoreceptors, called transducer-like proteins (Tlp), to colonize and persist within the stomach. TlpA has been implicated in sensing arginine, bicarbonate, and acid, but no experimentally determined protein structures of TlpA were available to better understand ligand binding and signal transduction. Here, we report three crystal structures of the periplasmic portion of TlpA, which contains tandem PAS/Cache domains, similar to a recently published structure of the lactate-sensing chemoreceptor TlpC from H. pylori. We performed sequence analyses to identify TlpA and TlpC homologues and used residue conservation amongst these homologues to implicate regions important for the general tandem PAS/Cache fold, and residues specific to TlpA function. Comparisons with TlpC show that despite high similarity across the general structure, TlpA lacks the residues required to bind lactate, and instead contains a pocket almost entirely hydrophobic in nature. This article is protected by copyright. All rights reserved. Structures of the Ligand-Binding Domain of Helicobacter pylori Chemoreceptor TlpA.,Sweeney EG, Perkins A, Kallio K, Remington SJ, Guillemin K Protein Sci. 2018 Aug 31. doi: 10.1002/pro.3503. PMID:30171638 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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