2qm4: Difference between revisions
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{{STRUCTURE_2qm4| PDB=2qm4 | SCENE= }} | |||
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'''Crystal structure of human XLF/Cernunnos, a non-homologous end-joining factor''' | '''Crystal structure of human XLF/Cernunnos, a non-homologous end-joining factor''' | ||
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[[Category: Li, Y.]] | [[Category: Li, Y.]] | ||
[[Category: Sibanda, B L.]] | [[Category: Sibanda, B L.]] | ||
[[Category: | [[Category: Beta-sandwich]] | ||
[[Category: | [[Category: Coiled-coil]] | ||
[[Category: | [[Category: Homodimer]] | ||
[[Category: | [[Category: Recombination]] | ||
[[Category: | [[Category: Xrcc4 like factor]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 15:11:22 2008'' | |||
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Revision as of 12:11, 4 May 2008
Crystal structure of human XLF/Cernunnos, a non-homologous end-joining factor
Overview
The recently characterised 299-residue human XLF/Cernunnos protein plays a crucial role in DNA repair by non-homologous end joining (NHEJ) and interacts with the XRCC4-DNA Ligase IV complex. Here, we report the crystal structure of the XLF (1-233) homodimer at 2.3 A resolution, confirming the predicted structural similarity to XRCC4. The XLF coiled-coil, however, is shorter than that of XRCC4 and undergoes an unexpected reverse in direction giving rise to a short distorted four helical bundle and a C-terminal helical structure wedged between the coiled-coil and head domain. The existence of a dimer as the major species is confirmed by size-exclusion chromatography, analytical ultracentrifugation, small-angle X-ray scattering and other biophysical methods. We show that the XLF structure is not easily compatible with a proposed XRCC4:XLF heterodimer. However, we demonstrate interactions between dimers of XLF and XRCC4 by surface plasmon resonance and analyse these in terms of surface properties, amino-acid conservation and mutations in immunodeficient patients. Our data are most consistent with head-to-head interactions in a 2:2:1 XRCC4:XLF:Ligase IV complex.
About this Structure
2QM4 is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Crystal structure of human XLF/Cernunnos reveals unexpected differences from XRCC4 with implications for NHEJ., Li Y, Chirgadze DY, Bolanos-Garcia VM, Sibanda BL, Davies OR, Ahnesorg P, Jackson SP, Blundell TL, EMBO J. 2008 Jan 9;27(1):290-300. Epub 2007 Nov 29. PMID:18046455 Page seeded by OCA on Sun May 4 15:11:22 2008
