6h8o: Difference between revisions
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==Crystal structure of the Master-Rep protein nuclease domain from the Faba Bean Necrotic Yellows Virus== | ==Crystal structure of the Master-Rep protein nuclease domain from the Faba Bean Necrotic Yellows Virus== | ||
<StructureSection load='6h8o' size='340' side='right' caption='[[6h8o]], [[Resolution|resolution]] 1.15Å' scene=''> | <StructureSection load='6h8o' size='340' side='right'caption='[[6h8o]], [[Resolution|resolution]] 1.15Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[6h8o]] is a 2 chain structure with sequence from [ | <table><tr><td colspan='2'>[[6h8o]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Faba_bean_necrotic_yellows_virus Faba bean necrotic yellows virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6H8O OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6H8O FirstGlance]. <br> | ||
</td></tr><tr id=' | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.15Å</td></tr> | ||
<tr id=' | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6h8o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6h8o OCA], [https://pdbe.org/6h8o PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6h8o RCSB], [https://www.ebi.ac.uk/pdbsum/6h8o PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6h8o ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | |||
[https://www.uniprot.org/uniprot/MREP_FBNY2 MREP_FBNY2] Essential for the replication of all genomic viral ssDNA (trans-replication). The closed circular ssDNA genome is first converted to a superhelical dsDNA. Rep binds a specific hairpin at the genome origin of replication. Introduces an endonucleolytic nick within the conserved sequence 5'-A[GT]TATTAC-3' in the intergenic region of the genome, thereby initiating the rolling circle replication (RCR). Following cleavage, binds covalently to the 5'-phosphate of DNA as a tyrosyl ester. The cleavage gives rise to a free 3'-OH that serves as a primer for the cellular DNA polymerase. The polymerase synthesizes the (+) strand DNA by rolling circle mechanism. After one round of replication, a Rep-catalyzed nucleotidyl transfer reaction releases a circular single-stranded virus genome, thereby terminating the replication. Displays origin-specific DNA cleavage, nucleotidyl transferase, ATPase and helicase activities. | |||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Faba bean necrotic yellows virus]] | [[Category: Faba bean necrotic yellows virus]] | ||
[[Category: | [[Category: Large Structures]] | ||
[[Category: | [[Category: Gonzalez-Montes L]] | ||
[[Category: | [[Category: Moncalian G]] | ||
